Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity
Abstract Background Semen is a critical vector for human immunodeficiency virus (HIV) sexual transmission and harbors seminal amyloid fibrils that can markedly enhance HIV infection. Semen-derived enhancer of viral infection (SEVI) is one of the best-characterized seminal amyloid fibrils. Due to the...
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doaj-3f22d797e13345f6ab49ae0e0338ece72020-11-24T21:30:32ZengBMCRetrovirology1742-46902018-07-0115112410.1186/s12977-018-0432-3Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activityRuxia Ren0Shuwen Yin1Baolong Lai2Lingzhen Ma3Jiayong Wen4Xuanxuan Zhang5Fangyuan Lai6Shuwen Liu7Lin Li8Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityDepartment of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key Laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical UniversityAbstract Background Semen is a critical vector for human immunodeficiency virus (HIV) sexual transmission and harbors seminal amyloid fibrils that can markedly enhance HIV infection. Semen-derived enhancer of viral infection (SEVI) is one of the best-characterized seminal amyloid fibrils. Due to their highly cationic properties, SEVI fibrils can capture HIV virions, increase viral attachment to target cells, and augment viral fusion. Some studies have reported that myricetin antagonizes amyloid β-protein (Aβ) formation; myricetin also displays strong anti-HIV activity in vitro. Results Here, we report that myricetin inhibits the formation of SEVI fibrils by binding to the amyloidogenic region of the SEVI precursor peptide (PAP248–286) and disrupting PAP248–286 oligomerization. In addition, myricetin was found to remodel preformed SEVI fibrils and to influence the activity of SEVI in promoting HIV-1 infection. Moreover, myricetin showed synergistic effects against HIV-1 infection in combination with other antiretroviral drugs in semen. Conclusions Incorporation of myricetin into a combination bifunctional microbicide with both anti-SEVI and anti-HIV activities is a highly promising approach to preventing sexual transmission of HIV.http://link.springer.com/article/10.1186/s12977-018-0432-3HIVMyricetinAmyloid fibrilsSEVISynergistic antiviral effects |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruxia Ren Shuwen Yin Baolong Lai Lingzhen Ma Jiayong Wen Xuanxuan Zhang Fangyuan Lai Shuwen Liu Lin Li |
spellingShingle |
Ruxia Ren Shuwen Yin Baolong Lai Lingzhen Ma Jiayong Wen Xuanxuan Zhang Fangyuan Lai Shuwen Liu Lin Li Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity Retrovirology HIV Myricetin Amyloid fibrils SEVI Synergistic antiviral effects |
author_facet |
Ruxia Ren Shuwen Yin Baolong Lai Lingzhen Ma Jiayong Wen Xuanxuan Zhang Fangyuan Lai Shuwen Liu Lin Li |
author_sort |
Ruxia Ren |
title |
Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity |
title_short |
Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity |
title_full |
Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity |
title_fullStr |
Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity |
title_full_unstemmed |
Myricetin antagonizes semen-derived enhancer of viral infection (SEVI) formation and influences its infection-enhancing activity |
title_sort |
myricetin antagonizes semen-derived enhancer of viral infection (sevi) formation and influences its infection-enhancing activity |
publisher |
BMC |
series |
Retrovirology |
issn |
1742-4690 |
publishDate |
2018-07-01 |
description |
Abstract Background Semen is a critical vector for human immunodeficiency virus (HIV) sexual transmission and harbors seminal amyloid fibrils that can markedly enhance HIV infection. Semen-derived enhancer of viral infection (SEVI) is one of the best-characterized seminal amyloid fibrils. Due to their highly cationic properties, SEVI fibrils can capture HIV virions, increase viral attachment to target cells, and augment viral fusion. Some studies have reported that myricetin antagonizes amyloid β-protein (Aβ) formation; myricetin also displays strong anti-HIV activity in vitro. Results Here, we report that myricetin inhibits the formation of SEVI fibrils by binding to the amyloidogenic region of the SEVI precursor peptide (PAP248–286) and disrupting PAP248–286 oligomerization. In addition, myricetin was found to remodel preformed SEVI fibrils and to influence the activity of SEVI in promoting HIV-1 infection. Moreover, myricetin showed synergistic effects against HIV-1 infection in combination with other antiretroviral drugs in semen. Conclusions Incorporation of myricetin into a combination bifunctional microbicide with both anti-SEVI and anti-HIV activities is a highly promising approach to preventing sexual transmission of HIV. |
topic |
HIV Myricetin Amyloid fibrils SEVI Synergistic antiviral effects |
url |
http://link.springer.com/article/10.1186/s12977-018-0432-3 |
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