Molecular Characterization of Fluoroquinolone-Resistant <i>Bartonella bacilliformis</i>

The presence of amino acid changes in GyrA, GyrB, ParC, ParE, and in a proposed chromosomal chloramphenicol acetyl transferase (CAT), as well as mutations at <i>23S rRNA</i>, were established by PCR and sequencing in 38 <i>B. bacilliformis</i> clinical isolates from four diff...

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Bibliographic Details
Main Authors: Giovanna Mendoza-Mujica, Diana Flores-León, Joaquim Ruiz
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Pathogens
Subjects:
Online Access:https://www.mdpi.com/2076-0817/10/7/876
Description
Summary:The presence of amino acid changes in GyrA, GyrB, ParC, ParE, and in a proposed chromosomal chloramphenicol acetyl transferase (CAT), as well as mutations at <i>23S rRNA</i>, were established by PCR and sequencing in 38 <i>B. bacilliformis</i> clinical isolates from four different areas in Peru. Eighteen out of 24 (75%) isolates showing ciprofloxacin resistance for both disk-diffusion and e-test presented amino acid substitutions in GyrA (G<sub>89</sub>C, six isolates, A<sub>91</sub>V, 1 isolate) GyrB (S<sub>474</sub>F, 10 isolates) or both (GyrA D<sub>95</sub>N and GyrB S<sub>474</sub>F, one isolate). Two out of 14 susceptible isolates presented amino acid substitutions at GyrB (S<sub>474</sub>F) or a double substitution GyrA D<sub>95</sub>N and GyrB S<sub>474</sub>F. Of note, ciprofloxacin-resistant isolates were recovered in the four areas studied. No amino acid change was observed at ParC or ParE. Only one isolate showed chloramphenicol resistance, but no alteration was present in either <i>23S rRNA</i> or CAT. <i>B. bacilliformis</i> resistant to quinolones are extended throughout Peru, with amino acid substitutions at GyrA or GyrB as the main, albeit not exclusive, cause. <i>B. bacilliformis</i> seems to have an apparent facility to develop mutations on GyrB outside the classical positions 91, 95 of GyrA and 85, 88 of ParC.
ISSN:2076-0817