Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha
The expression of bacterial gusA gene encoding for E. coli enzyme β-glucuronidase in haploid and diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha was studied. Haploid strains were transformed with plasmid carrying gusA gene under the control of strong alcohol oxidase (AOX) promot...
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2014-12-01
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doaj-3f2e5d242e8946a1bbbd88310858e6a62020-11-25T01:34:24ZengPlovdiv University Press Journal of BioScience and Biotechnology1314-62381314-62462014-12-0133247252Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorphaAnton Stoyanov0Penka Petrova1Kantcho Lahtchev2The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia; BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia; BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia; BulgariaThe expression of bacterial gusA gene encoding for E. coli enzyme β-glucuronidase in haploid and diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha was studied. Haploid strains were transformed with plasmid carrying gusA gene under the control of strong alcohol oxidase (AOX) promoter and several Gus+ transformants were isolated. They were crossed with wild type strains lacking Gus activity. The resulting diploids were sporulated and several meiotic segregants with increased Gus activity were isolated. Some of these segregants were crossed and the corresponding diploid strains were obtained. It was found that Gus activity of diploid strains was twice higher compared to haploids, whereas Gus activity of diploids was increased about six fold compared to those of haploids. Our results can be used as an approach for development of yeast strains with elevated expression of heterologous proteins.http://www.jbb.uni-plovdiv.bg/documents/27807/352488/jbb_2014-3(3)-pages_247-252.pdfOgataea (Hansenula) polymorphaβ-glucuronidasediploid strains |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anton Stoyanov Penka Petrova Kantcho Lahtchev |
spellingShingle |
Anton Stoyanov Penka Petrova Kantcho Lahtchev Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha Journal of BioScience and Biotechnology Ogataea (Hansenula) polymorpha β-glucuronidase diploid strains |
author_facet |
Anton Stoyanov Penka Petrova Kantcho Lahtchev |
author_sort |
Anton Stoyanov |
title |
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha |
title_short |
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha |
title_full |
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha |
title_fullStr |
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha |
title_full_unstemmed |
Enhanced heterologous gene expression in diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha |
title_sort |
enhanced heterologous gene expression in diploid cells of methylotrophic yeast hansenula (ogataea) polymorpha |
publisher |
Plovdiv University Press |
series |
Journal of BioScience and Biotechnology |
issn |
1314-6238 1314-6246 |
publishDate |
2014-12-01 |
description |
The expression of bacterial gusA gene encoding for E. coli enzyme β-glucuronidase in haploid and diploid cells of methylotrophic yeast Hansenula (Ogataea) polymorpha was studied. Haploid strains were transformed with plasmid carrying gusA gene under the control of strong alcohol oxidase (AOX) promoter and several Gus+ transformants were isolated. They were crossed with wild type strains lacking Gus activity. The resulting diploids were sporulated and several meiotic segregants with increased Gus activity were isolated. Some of these segregants were crossed and the corresponding diploid strains were obtained. It was found that Gus activity of diploid strains was twice higher compared to haploids, whereas Gus activity of diploids was increased about six fold compared to those of haploids. Our results can be used as an approach for development of yeast strains with elevated expression of heterologous proteins. |
topic |
Ogataea (Hansenula) polymorpha β-glucuronidase diploid strains |
url |
http://www.jbb.uni-plovdiv.bg/documents/27807/352488/jbb_2014-3(3)-pages_247-252.pdf |
work_keys_str_mv |
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