Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis.
Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elu...
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2020-05-01
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Series: | PLoS Pathogens |
Online Access: | https://doi.org/10.1371/journal.ppat.1008502 |
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doaj-3f3a7c79aec04b54a91e9e426787b3262021-04-21T17:14:19ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-05-01165e100850210.1371/journal.ppat.1008502Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis.Roghaiyeh SafariJean-Rock JacquesYves BrostauxLuc WillemsViruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elucidate the mechanisms associated with BLV non-coding RNAs, we performed phenotypic and transcriptomic analyzes in a reverse genetics system. RNA sequencing of B-lymphocytes revealed that cell proliferation is the most significant mechanism associated with ablation of the viral non-coding RNAs. To assess the biological relevance of this observation, we determined the cell kinetic parameters in vivo using intravenous injection of BrdU and CFSE. Fitting the data to a mathematical model provided the rates of cell proliferation and death. Our data show that deletion of miRNAs correlates with reduced proliferation of the infected cell and lack of pathogenesis.https://doi.org/10.1371/journal.ppat.1008502 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roghaiyeh Safari Jean-Rock Jacques Yves Brostaux Luc Willems |
spellingShingle |
Roghaiyeh Safari Jean-Rock Jacques Yves Brostaux Luc Willems Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. PLoS Pathogens |
author_facet |
Roghaiyeh Safari Jean-Rock Jacques Yves Brostaux Luc Willems |
author_sort |
Roghaiyeh Safari |
title |
Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. |
title_short |
Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. |
title_full |
Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. |
title_fullStr |
Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. |
title_full_unstemmed |
Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis. |
title_sort |
ablation of non-coding rnas affects bovine leukemia virus b lymphocyte proliferation and abrogates oncogenesis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2020-05-01 |
description |
Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elucidate the mechanisms associated with BLV non-coding RNAs, we performed phenotypic and transcriptomic analyzes in a reverse genetics system. RNA sequencing of B-lymphocytes revealed that cell proliferation is the most significant mechanism associated with ablation of the viral non-coding RNAs. To assess the biological relevance of this observation, we determined the cell kinetic parameters in vivo using intravenous injection of BrdU and CFSE. Fitting the data to a mathematical model provided the rates of cell proliferation and death. Our data show that deletion of miRNAs correlates with reduced proliferation of the infected cell and lack of pathogenesis. |
url |
https://doi.org/10.1371/journal.ppat.1008502 |
work_keys_str_mv |
AT roghaiyehsafari ablationofnoncodingrnasaffectsbovineleukemiavirusblymphocyteproliferationandabrogatesoncogenesis AT jeanrockjacques ablationofnoncodingrnasaffectsbovineleukemiavirusblymphocyteproliferationandabrogatesoncogenesis AT yvesbrostaux ablationofnoncodingrnasaffectsbovineleukemiavirusblymphocyteproliferationandabrogatesoncogenesis AT lucwillems ablationofnoncodingrnasaffectsbovineleukemiavirusblymphocyteproliferationandabrogatesoncogenesis |
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1714666371204251648 |