Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein.
The urinary metabolites cyanoethyl mercapturic acid (CEMA) and 3-hydroxypropyl mercapturic acid (3-HPMA) have been widely used as biomarkers of exposure to acrylonitrile and acrolein, respectively, but there are no published data on their consistency over time in the urine of cigarette smokers. We p...
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doaj-3f449759945b4aec8ffa07f558608c692021-03-03T21:33:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01141e021010410.1371/journal.pone.0210104Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein.Menglan ChenSteven G CarmellaChistopher SipeJoni JensenXianghua LuoChap T LeSharon E MurphyNeal L BenowitzF Joseph McClernonRyan VandreySharon S AllenRachel Denlinger-AptePaul M CinciripiniAndrew A StrasserMustafa al'AbsiJason D RobinsonEric C DonnyDorothy HatsukamiStephen S HechtThe urinary metabolites cyanoethyl mercapturic acid (CEMA) and 3-hydroxypropyl mercapturic acid (3-HPMA) have been widely used as biomarkers of exposure to acrylonitrile and acrolein, respectively, but there are no published data on their consistency over time in the urine of cigarette smokers. We provided, free of charge over a 20 week period, Spectrum NRC600/601 research cigarettes to cigarette smokers in the control arm of a randomized clinical trial of the reduced nicotine cigarette. Urine samples were collected at weeks 4, 8, 12, 16, and 20 and analyzed for CEMA and 3-HPMA, and total nicotine equivalents (TNE) using validated methods. Creatinine-corrected intra-class correlation coefficients for CEMA, 3-HPMA, and TNE were 0.67, 0.46, and 0.68, respectively, indicating good longitudinal consistency for CEMA, while that of 3-HPMA was fair. A strong correlation between CEMA and TNE values was observed. These data support the use of CEMA as a reliable biomarker of tobacco smoke exposure. This is the first report of the longitudinal stability of the biomarkers of acrylonitrile and acrolein exposure in smokers. The data indicate that CEMA, the biomarker of acrylonitrile exposure, is consistent over time in cigarette smokers, supporting its use. While 3-HPMA levels were less stable over time, this biomarker is nevertheless a useful monitor of human acrolein exposure because of its specificity to this toxicant.https://doi.org/10.1371/journal.pone.0210104 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Menglan Chen Steven G Carmella Chistopher Sipe Joni Jensen Xianghua Luo Chap T Le Sharon E Murphy Neal L Benowitz F Joseph McClernon Ryan Vandrey Sharon S Allen Rachel Denlinger-Apte Paul M Cinciripini Andrew A Strasser Mustafa al'Absi Jason D Robinson Eric C Donny Dorothy Hatsukami Stephen S Hecht |
spellingShingle |
Menglan Chen Steven G Carmella Chistopher Sipe Joni Jensen Xianghua Luo Chap T Le Sharon E Murphy Neal L Benowitz F Joseph McClernon Ryan Vandrey Sharon S Allen Rachel Denlinger-Apte Paul M Cinciripini Andrew A Strasser Mustafa al'Absi Jason D Robinson Eric C Donny Dorothy Hatsukami Stephen S Hecht Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. PLoS ONE |
author_facet |
Menglan Chen Steven G Carmella Chistopher Sipe Joni Jensen Xianghua Luo Chap T Le Sharon E Murphy Neal L Benowitz F Joseph McClernon Ryan Vandrey Sharon S Allen Rachel Denlinger-Apte Paul M Cinciripini Andrew A Strasser Mustafa al'Absi Jason D Robinson Eric C Donny Dorothy Hatsukami Stephen S Hecht |
author_sort |
Menglan Chen |
title |
Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
title_short |
Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
title_full |
Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
title_fullStr |
Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
title_full_unstemmed |
Longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
title_sort |
longitudinal stability in cigarette smokers of urinary biomarkers of exposure to the toxicants acrylonitrile and acrolein. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
The urinary metabolites cyanoethyl mercapturic acid (CEMA) and 3-hydroxypropyl mercapturic acid (3-HPMA) have been widely used as biomarkers of exposure to acrylonitrile and acrolein, respectively, but there are no published data on their consistency over time in the urine of cigarette smokers. We provided, free of charge over a 20 week period, Spectrum NRC600/601 research cigarettes to cigarette smokers in the control arm of a randomized clinical trial of the reduced nicotine cigarette. Urine samples were collected at weeks 4, 8, 12, 16, and 20 and analyzed for CEMA and 3-HPMA, and total nicotine equivalents (TNE) using validated methods. Creatinine-corrected intra-class correlation coefficients for CEMA, 3-HPMA, and TNE were 0.67, 0.46, and 0.68, respectively, indicating good longitudinal consistency for CEMA, while that of 3-HPMA was fair. A strong correlation between CEMA and TNE values was observed. These data support the use of CEMA as a reliable biomarker of tobacco smoke exposure. This is the first report of the longitudinal stability of the biomarkers of acrylonitrile and acrolein exposure in smokers. The data indicate that CEMA, the biomarker of acrylonitrile exposure, is consistent over time in cigarette smokers, supporting its use. While 3-HPMA levels were less stable over time, this biomarker is nevertheless a useful monitor of human acrolein exposure because of its specificity to this toxicant. |
url |
https://doi.org/10.1371/journal.pone.0210104 |
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