Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant

An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochrom...

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Main Authors: Cesar Koppe Grisolia, Adriano Barros Barata de Oliveira, Helder Bonfim, Maria de Nazaré Klautau-Guimarães
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2005-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026
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spelling doaj-3f467d6876024601abb822dee0a115922020-11-25T02:31:30ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852005-01-0128233433810.1590/S1415-47572005000200026Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plantCesar Koppe GrisoliaAdriano Barros Barata de OliveiraHelder BonfimMaria de Nazaré Klautau-GuimarãesAn evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei analysis, blood samples were drawn after 7, 21, 60, 102 and 142 days, respectively, in controlled conditions. The numbers of aberrant cells found in the Allium test did not differ among the four stages tested, nor when compared with the control. At all stages, the most concentrated sample was more toxic than the respective diluted samples, as demonstrated by the decreased mitotic index. Different sampling of the same group of fish revealed no increase in the micronuclei frequency, regardless of the period of exposure. A correlation between long-term exposure and micronuclei induction was not found either.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026Allium cepabiomonitoringdomestic sewagefishmicronucleimutagenicity
collection DOAJ
language English
format Article
sources DOAJ
author Cesar Koppe Grisolia
Adriano Barros Barata de Oliveira
Helder Bonfim
Maria de Nazaré Klautau-Guimarães
spellingShingle Cesar Koppe Grisolia
Adriano Barros Barata de Oliveira
Helder Bonfim
Maria de Nazaré Klautau-Guimarães
Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
Genetics and Molecular Biology
Allium cepa
biomonitoring
domestic sewage
fish
micronuclei
mutagenicity
author_facet Cesar Koppe Grisolia
Adriano Barros Barata de Oliveira
Helder Bonfim
Maria de Nazaré Klautau-Guimarães
author_sort Cesar Koppe Grisolia
title Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
title_short Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
title_full Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
title_fullStr Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
title_full_unstemmed Genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
title_sort genotoxicity evaluation of domestic sewage in a municipal wastewater treatment plant
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2005-01-01
description An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei analysis, blood samples were drawn after 7, 21, 60, 102 and 142 days, respectively, in controlled conditions. The numbers of aberrant cells found in the Allium test did not differ among the four stages tested, nor when compared with the control. At all stages, the most concentrated sample was more toxic than the respective diluted samples, as demonstrated by the decreased mitotic index. Different sampling of the same group of fish revealed no increase in the micronuclei frequency, regardless of the period of exposure. A correlation between long-term exposure and micronuclei induction was not found either.
topic Allium cepa
biomonitoring
domestic sewage
fish
micronuclei
mutagenicity
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026
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AT helderbonfim genotoxicityevaluationofdomesticsewageinamunicipalwastewatertreatmentplant
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