Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model
This study evaluated the biocompatibility and biological performance of novel additive-manufactured bioabsorbable iron-based porous suture anchors (iron_SAs). Two types of bioabsorbable iron_SAs, with double- and triple-helical structures (iron_SA_2_helix and iron_SA_3_helix, respectively), were com...
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doaj-3f67ca6cd64a4213b4c6bbf95ed1c0f02021-07-23T13:45:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-01227368736810.3390/ijms22147368Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit ModelChien-Cheng Tai0Hon-Lok Lo1Chen-Kun Liaw2Yu-Min Huang3Yen-Hua Huang4Kuo-Yi Yang5Chih-Chieh Huang6Shin-I Huang7Hsin-Hsin Shen8Tzu-Hung Lin9Chun-Kuan Lu10Wen-Chih Liu11Jui-Sheng Sun12Pei-I Tsai13Chih-Yu Chen14Internal Ph.D. Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Orthopedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanInternal Ph.D. Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanBiomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Chutung, Hsinchu 310401, TaiwanBiomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Chutung, Hsinchu 310401, TaiwanBiomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Chutung, Hsinchu 310401, TaiwanBiomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Chutung, Hsinchu 310401, TaiwanMaterial and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 31040, TaiwanDepartment of Orthopaedics, Park One International Hospital, Kaohsiung 813322, TaiwanDepartment of Orthopedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, TaiwanDepartment of Orthopedics, China Medical University, Taichung 40202, TaiwanBiomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Chutung, Hsinchu 310401, TaiwanDepartment of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, TaiwanThis study evaluated the biocompatibility and biological performance of novel additive-manufactured bioabsorbable iron-based porous suture anchors (iron_SAs). Two types of bioabsorbable iron_SAs, with double- and triple-helical structures (iron_SA_2_helix and iron_SA_3_helix, respectively), were compared with the synthetic polymer-based bioabsorbable suture anchor (polymer_SAs). An in vitro mechanical test, MTT assay, and scanning electron microscope (SEM) analysis were performed. An in vivo animal study was also performed. The three types of suture anchors were randomly implanted in the outer cortex of the lateral femoral condyle. The ultimate in vitro pullout strength of the iron_SA_3_helix group was significantly higher than the iron_SA_2_helix and polymer_SA groups. The MTT assay findings demonstrated no significant cytotoxicity, and the SEM analysis showed cells attachment on implant surface. The ultimate failure load of the iron_SA_3_helix group was significantly higher than that of the polymer_SA group. The micro-CT analysis indicated the iron_SA_3_helix group showed a higher bone volume fraction (BV/TV) after surgery. Moreover, both iron SAs underwent degradation with time. Iron_SAs with triple-helical threads and a porous structure demonstrated better mechanical strength and high biocompatibility after short-term implantation. The combined advantages of the mechanical superiority of the iron metal and the possibility of absorption after implantation make the iron_SA a suitable candidate for further development.https://www.mdpi.com/1422-0067/22/14/7368additive manufacturing (3D printing)bioabsorbableiron-basedsuture anchor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chien-Cheng Tai Hon-Lok Lo Chen-Kun Liaw Yu-Min Huang Yen-Hua Huang Kuo-Yi Yang Chih-Chieh Huang Shin-I Huang Hsin-Hsin Shen Tzu-Hung Lin Chun-Kuan Lu Wen-Chih Liu Jui-Sheng Sun Pei-I Tsai Chih-Yu Chen |
spellingShingle |
Chien-Cheng Tai Hon-Lok Lo Chen-Kun Liaw Yu-Min Huang Yen-Hua Huang Kuo-Yi Yang Chih-Chieh Huang Shin-I Huang Hsin-Hsin Shen Tzu-Hung Lin Chun-Kuan Lu Wen-Chih Liu Jui-Sheng Sun Pei-I Tsai Chih-Yu Chen Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model International Journal of Molecular Sciences additive manufacturing (3D printing) bioabsorbable iron-based suture anchor |
author_facet |
Chien-Cheng Tai Hon-Lok Lo Chen-Kun Liaw Yu-Min Huang Yen-Hua Huang Kuo-Yi Yang Chih-Chieh Huang Shin-I Huang Hsin-Hsin Shen Tzu-Hung Lin Chun-Kuan Lu Wen-Chih Liu Jui-Sheng Sun Pei-I Tsai Chih-Yu Chen |
author_sort |
Chien-Cheng Tai |
title |
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model |
title_short |
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model |
title_full |
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model |
title_fullStr |
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model |
title_full_unstemmed |
Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model |
title_sort |
biocompatibility and biological performance evaluation of additive-manufactured bioabsorbable iron-based porous suture anchor in a rabbit model |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-07-01 |
description |
This study evaluated the biocompatibility and biological performance of novel additive-manufactured bioabsorbable iron-based porous suture anchors (iron_SAs). Two types of bioabsorbable iron_SAs, with double- and triple-helical structures (iron_SA_2_helix and iron_SA_3_helix, respectively), were compared with the synthetic polymer-based bioabsorbable suture anchor (polymer_SAs). An in vitro mechanical test, MTT assay, and scanning electron microscope (SEM) analysis were performed. An in vivo animal study was also performed. The three types of suture anchors were randomly implanted in the outer cortex of the lateral femoral condyle. The ultimate in vitro pullout strength of the iron_SA_3_helix group was significantly higher than the iron_SA_2_helix and polymer_SA groups. The MTT assay findings demonstrated no significant cytotoxicity, and the SEM analysis showed cells attachment on implant surface. The ultimate failure load of the iron_SA_3_helix group was significantly higher than that of the polymer_SA group. The micro-CT analysis indicated the iron_SA_3_helix group showed a higher bone volume fraction (BV/TV) after surgery. Moreover, both iron SAs underwent degradation with time. Iron_SAs with triple-helical threads and a porous structure demonstrated better mechanical strength and high biocompatibility after short-term implantation. The combined advantages of the mechanical superiority of the iron metal and the possibility of absorption after implantation make the iron_SA a suitable candidate for further development. |
topic |
additive manufacturing (3D printing) bioabsorbable iron-based suture anchor |
url |
https://www.mdpi.com/1422-0067/22/14/7368 |
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