Uptake and subcellular distribution of cadmium in <i>Datura stramonium</i> L.

• Main Authors: ZHOU Zhen, YANG Su-qin, LIU Hai-tao, CHEN Xian-feng, CHEN Xin, ZHANG Biao
• Format: Article
• Language: zho
• Published: Agro-Environmental Protection Institute, Ministry of Agriculture 2019-05-01
• Series: Journal of Agricultural Resources and Environment
• Subjects: <i>Datura stramonium</i> L, Cd accumulation, Cd²⁺ flux, subcellular distribution, transfer factor
• Online Access: http://www.aed.org.cn/nyzyyhjxb/html/2019/3/20190317.htm
• ISSN: 2095-6819; 2095-6819

To study the tolerance of <i>Datura stramonium</i> L. to Cd, previously selected <i>D.stramonium</i> was used to evaluate Cd uptake and subcellular distribution under Cd stress using ahydroponic experiment. The results showed that the Cd concentration among different parts of <i>D.stramonium</i> decreased in the following order at both low and high Cd concentrations in medium:Root > stem > leaf. Nevertheless, there was no significant difference in the transfer factor. Cd²⁺ flux was significantly different in different zones of the root surface; the flux of Cd²⁺ in the meristematic and elongation zones was significantly higher than that in the root cap and maturity zone. When the Cd concentration increased from 0.1 mg&#183;L^-1 to 2.5 mg&#183;L^-1, the proportion of Cd in cell walls and the soluble fraction increased. Thus, the uptake of Cd by the roots of <i>D.stramonium</i> occurred mainly within the meristematic and elongation zones. When the Cd concentration in medium was low, restriction of Cd transport by cell walls in the roots and compartmentalization by the soluble fraction of cells in the shoots played a vital role in Cd detoxification. When the Cd concentration was high, the proportion of Cd in the soluble fraction of root cells increased, and Cd could migrate to the leaves and stems. Therefore, enhanced Cd immobilization by cell walls in the shoots might serve as a mechanism of Cd tolerance in <i>D.stramonium</i>.