The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae
Cerambycidae is one of the most diversified groups within Coleoptera and includes nearly 35,000 known species. The relationships at the subfamily level within Cerambycidae have not been convincingly demonstrated and the gene rearrangement of mitochondrial genomes in Cerambycidae remains unclear due...
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doaj-3f70efdb8e2248f8966947ba512d866c2020-11-25T01:24:45ZengPeerJ Inc.PeerJ2167-83592019-09-017e763310.7717/peerj.7633The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within CerambycidaeJun Wang0Xin-Yi Dai1Xiao-Dong Xu2Zi-Yi Zhang3Dan-Na Yu4Kenneth B. Storey5Jia-Yong Zhang6College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaDepartment of Biology, Carleton University, Ottawa, Ontario, CanadaCollege of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang, ChinaCerambycidae is one of the most diversified groups within Coleoptera and includes nearly 35,000 known species. The relationships at the subfamily level within Cerambycidae have not been convincingly demonstrated and the gene rearrangement of mitochondrial genomes in Cerambycidae remains unclear due to the low numbers of sequenced mitogenomes. In the present study, we determined five complete mitogenomes of Cerambycidae and investigated the phylogenetic relationship among the subfamilies of Cerambycidae based on mitogenomes. The mitogenomic arrangement of all five species was identical to the ancestral Cerambycidae type without gene rearrangement. Remarkably, however, two large intergenic spacers were detected in the mitogenome of Pterolophia sp. ZJY-2019. The origins of these intergenic spacers could be explained by the slipped-strand mispairing and duplication/random loss models. A conserved motif was found between trnS2 and nad1 gene, which was proposed to be a binding site of a transcription termination peptide. Also, tandem repeat units were identified in the A + T-rich region of all five mitogenomes. The monophyly of Lamiinae and Prioninae was strongly supported by both MrBayes and RAxML analyses based on nucleotide datasets, whereas the Cerambycinae and Lepturinae were recovered as non-monophyletic.https://peerj.com/articles/7633.pdfMitochondrial genomeCerambycidaeIntergenic spacerPhylogenetic relationship |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Wang Xin-Yi Dai Xiao-Dong Xu Zi-Yi Zhang Dan-Na Yu Kenneth B. Storey Jia-Yong Zhang |
spellingShingle |
Jun Wang Xin-Yi Dai Xiao-Dong Xu Zi-Yi Zhang Dan-Na Yu Kenneth B. Storey Jia-Yong Zhang The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae PeerJ Mitochondrial genome Cerambycidae Intergenic spacer Phylogenetic relationship |
author_facet |
Jun Wang Xin-Yi Dai Xiao-Dong Xu Zi-Yi Zhang Dan-Na Yu Kenneth B. Storey Jia-Yong Zhang |
author_sort |
Jun Wang |
title |
The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae |
title_short |
The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae |
title_full |
The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae |
title_fullStr |
The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae |
title_full_unstemmed |
The complete mitochondrial genomes of five longicorn beetles (Coleoptera: Cerambycidae) and phylogenetic relationships within Cerambycidae |
title_sort |
complete mitochondrial genomes of five longicorn beetles (coleoptera: cerambycidae) and phylogenetic relationships within cerambycidae |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2019-09-01 |
description |
Cerambycidae is one of the most diversified groups within Coleoptera and includes nearly 35,000 known species. The relationships at the subfamily level within Cerambycidae have not been convincingly demonstrated and the gene rearrangement of mitochondrial genomes in Cerambycidae remains unclear due to the low numbers of sequenced mitogenomes. In the present study, we determined five complete mitogenomes of Cerambycidae and investigated the phylogenetic relationship among the subfamilies of Cerambycidae based on mitogenomes. The mitogenomic arrangement of all five species was identical to the ancestral Cerambycidae type without gene rearrangement. Remarkably, however, two large intergenic spacers were detected in the mitogenome of Pterolophia sp. ZJY-2019. The origins of these intergenic spacers could be explained by the slipped-strand mispairing and duplication/random loss models. A conserved motif was found between trnS2 and nad1 gene, which was proposed to be a binding site of a transcription termination peptide. Also, tandem repeat units were identified in the A + T-rich region of all five mitogenomes. The monophyly of Lamiinae and Prioninae was strongly supported by both MrBayes and RAxML analyses based on nucleotide datasets, whereas the Cerambycinae and Lepturinae were recovered as non-monophyletic. |
topic |
Mitochondrial genome Cerambycidae Intergenic spacer Phylogenetic relationship |
url |
https://peerj.com/articles/7633.pdf |
work_keys_str_mv |
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