RIG-I and dsRNA-induced IFNbeta activation.
Except for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immu...
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doaj-3f76c757d86644fd9f330466bbae2d1b2020-11-24T22:06:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01312e396510.1371/journal.pone.0003965RIG-I and dsRNA-induced IFNbeta activation.Stéphane HausmannJean-Baptiste MarqCaroline TapparelDaniel KolakofskyDominique GarcinExcept for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immune response, whereas picornaviruses require mda-5. We have examined a SeV infection whose ability to induce interferon depends on the generation of capped dsRNA (without free 5' tri-phosphate ends), and found that this infection as well requires RIG-I and not mda-5. We also provide evidence that RIG-I interacts with poly-I/C in vivo, and that heteropolymeric dsRNA and poly-I/C interact directly with RIG-I in vitro, but in different ways; i.e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain.http://europepmc.org/articles/PMC2607022?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stéphane Hausmann Jean-Baptiste Marq Caroline Tapparel Daniel Kolakofsky Dominique Garcin |
spellingShingle |
Stéphane Hausmann Jean-Baptiste Marq Caroline Tapparel Daniel Kolakofsky Dominique Garcin RIG-I and dsRNA-induced IFNbeta activation. PLoS ONE |
author_facet |
Stéphane Hausmann Jean-Baptiste Marq Caroline Tapparel Daniel Kolakofsky Dominique Garcin |
author_sort |
Stéphane Hausmann |
title |
RIG-I and dsRNA-induced IFNbeta activation. |
title_short |
RIG-I and dsRNA-induced IFNbeta activation. |
title_full |
RIG-I and dsRNA-induced IFNbeta activation. |
title_fullStr |
RIG-I and dsRNA-induced IFNbeta activation. |
title_full_unstemmed |
RIG-I and dsRNA-induced IFNbeta activation. |
title_sort |
rig-i and dsrna-induced ifnbeta activation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-01-01 |
description |
Except for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immune response, whereas picornaviruses require mda-5. We have examined a SeV infection whose ability to induce interferon depends on the generation of capped dsRNA (without free 5' tri-phosphate ends), and found that this infection as well requires RIG-I and not mda-5. We also provide evidence that RIG-I interacts with poly-I/C in vivo, and that heteropolymeric dsRNA and poly-I/C interact directly with RIG-I in vitro, but in different ways; i.e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain. |
url |
http://europepmc.org/articles/PMC2607022?pdf=render |
work_keys_str_mv |
AT stephanehausmann rigianddsrnainducedifnbetaactivation AT jeanbaptistemarq rigianddsrnainducedifnbetaactivation AT carolinetapparel rigianddsrnainducedifnbetaactivation AT danielkolakofsky rigianddsrnainducedifnbetaactivation AT dominiquegarcin rigianddsrnainducedifnbetaactivation |
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