Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology
ABSTRACT: Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae Folium.Methods Response surface methodology (RSM), combining Plackett–Burman design (PBD), steepest ascent method, and Box–Behnken design (BBD), was employed. The significant fac...
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KeAi Communications Co., Ltd.
2018-09-01
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Series: | Digital Chinese Medicine |
Online Access: | http://www.sciencedirect.com/science/article/pii/S258937771930031X |
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DOAJ |
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English |
format |
Article |
sources |
DOAJ |
author |
Ling-Jia ZHAO Bo-Hou XIA Li-Mei LIN Su-Hui XIONG Jie TANG Duan-Fang LIAO |
spellingShingle |
Ling-Jia ZHAO Bo-Hou XIA Li-Mei LIN Su-Hui XIONG Jie TANG Duan-Fang LIAO Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology Digital Chinese Medicine |
author_facet |
Ling-Jia ZHAO Bo-Hou XIA Li-Mei LIN Su-Hui XIONG Jie TANG Duan-Fang LIAO |
author_sort |
Ling-Jia ZHAO |
title |
Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology |
title_short |
Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology |
title_full |
Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology |
title_fullStr |
Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology |
title_full_unstemmed |
Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology |
title_sort |
optimization of ultrasound-assisted enzymatic polysaccharide extraction from turpiniae folium based on response surface methodology |
publisher |
KeAi Communications Co., Ltd. |
series |
Digital Chinese Medicine |
issn |
2589-3777 |
publishDate |
2018-09-01 |
description |
ABSTRACT: Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae Folium.Methods Response surface methodology (RSM), combining Plackett–Burman design (PBD), steepest ascent method, and Box–Behnken design (BBD), was employed. The significant factors contributing to polysaccharide production were determined by PBD. The path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum region. BBD and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal values.Results The significant factors contributing to polysaccharide production were enzyme concentration, extraction time, and liquid-to-solid ratio. The optimal conditions for maximal TFP were: enzyme concentration of 2.8 %, extraction time of 41 min, and liquid-to-solid ratio of 27 mL/g. The experimental yield of 3.08% was in good agreement with the model-predicted yield of 3.1% under the optimized conditions.Conclusion This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide, which can contribute to its further production and application. Keywords: Optimization Polysaccharides, Turpinia argute, Turpiniae Folium, Response surface methodology |
url |
http://www.sciencedirect.com/science/article/pii/S258937771930031X |
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doaj-3f97734e6b724bf399dd13080addc42f2020-11-25T03:48:44ZengKeAi Communications Co., Ltd.Digital Chinese Medicine2589-37772018-09-0113239246Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface MethodologyLing-Jia ZHAO0Bo-Hou XIA1Li-Mei LIN2Su-Hui XIONG3Jie TANG4Duan-Fang LIAO5College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaCollege of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Key laboratory for quality of bulk herbs for Hunan Province, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Corresponding author: Duan-Fang LIAO, Professor, M.D., Ph.D.. Research direction: Cardiovascular pharmacology. E-mail: dfliao@hnucm.edu.cn. Peer review under the responsibility of Hunan University of Chinese Medicine.ABSTRACT: Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae Folium.Methods Response surface methodology (RSM), combining Plackett–Burman design (PBD), steepest ascent method, and Box–Behnken design (BBD), was employed. The significant factors contributing to polysaccharide production were determined by PBD. The path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum region. BBD and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal values.Results The significant factors contributing to polysaccharide production were enzyme concentration, extraction time, and liquid-to-solid ratio. The optimal conditions for maximal TFP were: enzyme concentration of 2.8 %, extraction time of 41 min, and liquid-to-solid ratio of 27 mL/g. The experimental yield of 3.08% was in good agreement with the model-predicted yield of 3.1% under the optimized conditions.Conclusion This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide, which can contribute to its further production and application. Keywords: Optimization Polysaccharides, Turpinia argute, Turpiniae Folium, Response surface methodologyhttp://www.sciencedirect.com/science/article/pii/S258937771930031X |