Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks?
The brain and subesophageal ganglion (BR-SG) of the commercial silk worm, Bombyx mori, were stained immunohistochemically at the larval stage for circadian clock neurons with antibodies against Doubletime (DBT) of B. mori and Period (PER) of Periplaneta americana. The BR-SGs were also stained with a...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2003-06-01
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doaj-3fab55439f074aa19e54a254d495652c2021-04-16T20:33:32ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292003-06-01100228328610.14411/eje.2003.044eje-200302-0009Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks?Shao QI-MIAO0Seiji TANAKA1Makio TAKEDA2Graduate School of Science and Technology, Kobe University, Rokkoda-cho, Nada-ku, Kobe 657-8501 JapanLaboratory of Insect Life-cycle and Physiology, Institute of Insect and Animal Sciences, Owashi, Tsukuba, 305-8634, JapanGraduate School of Science and Technology, Kobe University, Rokkoda-cho, Nada-ku, Kobe 657-8501 JapanThe brain and subesophageal ganglion (BR-SG) of the commercial silk worm, Bombyx mori, were stained immunohistochemically at the larval stage for circadian clock neurons with antibodies against Doubletime (DBT) of B. mori and Period (PER) of Periplaneta americana. The BR-SGs were also stained with antisera against [Arg7]-corazonin, which has been known to be present in B. mori and co-localized with PER in Manduca sexta, and against [His7]-corazonin, a homolog identified in other species. From co-localization of [Arg7]-corazonin and PER-like reactivities in the pars lateralis, [Arg7]-corazonin is suspected to be a downstream regulator of the circadian clock in M. sexta. DBT- and corazonin-like immunohistochemical reactivities were found in both the neurosecretory cells of the pars intercerebralis (PIC) and pars lateralis (PL) in B. mori. Small numbers of neurons shared both reactivities against anti-DBT and anti-corazonin. The majority of the immunopositive cells were common to both corazonins, but some cells were unique in expressing either reactivity against [His7]-corazonin or [Arg7]-corazonin only. The results suggest that there is a diversity in the clock output pathway among lepidopterans and that [His7]-corazonin may be present in B. mori, as well as [Arg7]-corazonin, although the former has not been chemically identified in this species. Corazonin may be a downstream regulator of circadian clocks in B. mori because of the co-localization of [His7]-corazonin at PIC and [Arg7]-corazonin at PL with anti-DBT.https://www.eje.cz/artkey/eje-200302-0009_Immunohistochemical_localization_of_clock_proteins_DBT_and_PER_and_His7_-_and_Arg7_-corazonins_in_the_cere.phpdouble time (dbt)[his7]-corazonin[arg7]-corazoninbombyx moricircadian oscillation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shao QI-MIAO Seiji TANAKA Makio TAKEDA |
spellingShingle |
Shao QI-MIAO Seiji TANAKA Makio TAKEDA Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? European Journal of Entomology double time (dbt) [his7]-corazonin [arg7]-corazonin bombyx mori circadian oscillation |
author_facet |
Shao QI-MIAO Seiji TANAKA Makio TAKEDA |
author_sort |
Shao QI-MIAO |
title |
Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? |
title_short |
Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? |
title_full |
Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? |
title_fullStr |
Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? |
title_full_unstemmed |
Immunohistochemical localization of clock proteins (DBT and PER), and [His7]- and [Arg7]-corazonins in the cerebral ganglia of Bombyx mori: Are corazonins downstream regulators of circadian clocks? |
title_sort |
immunohistochemical localization of clock proteins (dbt and per), and [his7]- and [arg7]-corazonins in the cerebral ganglia of bombyx mori: are corazonins downstream regulators of circadian clocks? |
publisher |
Institute of Entomology, Biology Centre, Czech Academy of Science |
series |
European Journal of Entomology |
issn |
1210-5759 1802-8829 |
publishDate |
2003-06-01 |
description |
The brain and subesophageal ganglion (BR-SG) of the commercial silk worm, Bombyx mori, were stained immunohistochemically at the larval stage for circadian clock neurons with antibodies against Doubletime (DBT) of B. mori and Period (PER) of Periplaneta americana. The BR-SGs were also stained with antisera against [Arg7]-corazonin, which has been known to be present in B. mori and co-localized with PER in Manduca sexta, and against [His7]-corazonin, a homolog identified in other species. From co-localization of [Arg7]-corazonin and PER-like reactivities in the pars lateralis, [Arg7]-corazonin is suspected to be a downstream regulator of the circadian clock in M. sexta. DBT- and corazonin-like immunohistochemical reactivities were found in both the neurosecretory cells of the pars intercerebralis (PIC) and pars lateralis (PL) in B. mori. Small numbers of neurons shared both reactivities against anti-DBT and anti-corazonin. The majority of the immunopositive cells were common to both corazonins, but some cells were unique in expressing either reactivity against [His7]-corazonin or [Arg7]-corazonin only. The results suggest that there is a diversity in the clock output pathway among lepidopterans and that [His7]-corazonin may be present in B. mori, as well as [Arg7]-corazonin, although the former has not been chemically identified in this species. Corazonin may be a downstream regulator of circadian clocks in B. mori because of the co-localization of [His7]-corazonin at PIC and [Arg7]-corazonin at PL with anti-DBT. |
topic |
double time (dbt) [his7]-corazonin [arg7]-corazonin bombyx mori circadian oscillation |
url |
https://www.eje.cz/artkey/eje-200302-0009_Immunohistochemical_localization_of_clock_proteins_DBT_and_PER_and_His7_-_and_Arg7_-corazonins_in_the_cere.php |
work_keys_str_mv |
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