Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

Summary: Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to so...

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Bibliographic Details
Main Authors: Zhongfu Ying, Zihuang Liu, Ge Xiang, Yanmin Xin, Junwei Wang, Xingguo Liu
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Flow Cytometry/Mass Cytometry
Metabolism
Microscopy
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002756
Description
Summary:Summary: Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high and low mPTP opening somatic cells by calcein fluorescence and reprogram these sorted cells to iPSCs. These protocols are also suitable for imaging mPTP opening and uncovering the mechanisms of mPTP function in other cell fate conversions.For complete details on the use and execution of this protocol, please refer to Ying et al. (2018).
ISSN:2666-1667

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