Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation
Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and pro-infla...
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doaj-3fbb8436db2e4fbf81856b3acd8ea4012020-11-24T22:21:38ZengElsevierJournal of Nutrition & Intermediary Metabolism2352-38592018-03-0111C1810.1016/j.jnim.2017.11.002Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammationSonni-Ali Miller0Jason A. White1Rupak Chowdhury2Dominique N. Gales3Berhanu Tameru4Amit K. Tiwari5Temesgen Samuel6Department of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDepartment of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDepartment of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDepartment of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDepartment of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDepartment of Pharmacology & Experimental Therapeutics, College of Pharmacy & Pharmaceutical Sciences, Frederic and Mary Wolfe Center, The University of Toledo – Health Science Campus, 3000 Arlington Ave., MS 1015, Toledo, OH 43614, United StatesDepartment of Pathobiology, Tuskegee University, College of Veterinary Medicine, 1200 W. Montgomery Road, Tuskegee AL 36088, United StatesDietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and pro-inflammatory cytokine secretion to test the hypothesis that oral consumption of whole grape formulation reduces inflammatory signaling in the body. NF-κB luciferase reporter mice were divided into two groups, one which was fed an experimental diet formulated with 4% (w/w) whole grape powder (WGP) or another which was fed a control diet formulated with 3.6% glucose/fructose (w/w) combination. Simulated inflammation was induced in the mice by intraperitoneal injection of lipopolysaccharide (LPS). In vivo imaging was used to determine the effect of each diet on NF-κB activity. We found that there were no significant differences in weight gain between the WGP and control diet groups. However, there was a statistically significant (p < 0.0001) difference in the progression of basal levels of NF-κB signaling between mice fed on control or WGP diet. There were no significant differences in NF-κB reporter indices between WGP- and control-diet groups after either acute or repeated inflammatory challenge. However, terminal blood collection revealed significantly (p < 0.01) lower serum concentrations of the inflammatory cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor alpha (TNFα) only among WGP diet mice subjected to acute inflammatory challenge. Overall, these data suggest that while diets supplemented with WGP may suppress steady-state low levels of inflammatory signaling, such a supplementation may not alleviate exogenously induced massive NF-κB activation.http://www.sciencedirect.com/science/article/pii/S2352385917302542Whole grape powderInflammationNF-kBDietIn vivoMouse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sonni-Ali Miller Jason A. White Rupak Chowdhury Dominique N. Gales Berhanu Tameru Amit K. Tiwari Temesgen Samuel |
spellingShingle |
Sonni-Ali Miller Jason A. White Rupak Chowdhury Dominique N. Gales Berhanu Tameru Amit K. Tiwari Temesgen Samuel Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation Journal of Nutrition & Intermediary Metabolism Whole grape powder Inflammation NF-kB Diet In vivo Mouse |
author_facet |
Sonni-Ali Miller Jason A. White Rupak Chowdhury Dominique N. Gales Berhanu Tameru Amit K. Tiwari Temesgen Samuel |
author_sort |
Sonni-Ali Miller |
title |
Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation |
title_short |
Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation |
title_full |
Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation |
title_fullStr |
Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation |
title_full_unstemmed |
Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation |
title_sort |
effects of consumption of whole grape powder on basal nf-κb signaling and inflammatory cytokine secretion in a mouse model of inflammation |
publisher |
Elsevier |
series |
Journal of Nutrition & Intermediary Metabolism |
issn |
2352-3859 |
publishDate |
2018-03-01 |
description |
Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and pro-inflammatory cytokine secretion to test the hypothesis that oral consumption of whole grape formulation reduces inflammatory signaling in the body. NF-κB luciferase reporter mice were divided into two groups, one which was fed an experimental diet formulated with 4% (w/w) whole grape powder (WGP) or another which was fed a control diet formulated with 3.6% glucose/fructose (w/w) combination. Simulated inflammation was induced in the mice by intraperitoneal injection of lipopolysaccharide (LPS). In vivo imaging was used to determine the effect of each diet on NF-κB activity. We found that there were no significant differences in weight gain between the WGP and control diet groups. However, there was a statistically significant (p < 0.0001) difference in the progression of basal levels of NF-κB signaling between mice fed on control or WGP diet. There were no significant differences in NF-κB reporter indices between WGP- and control-diet groups after either acute or repeated inflammatory challenge. However, terminal blood collection revealed significantly (p < 0.01) lower serum concentrations of the inflammatory cytokines Interleukin-6 (IL-6) and Tumor Necrosis Factor alpha (TNFα) only among WGP diet mice subjected to acute inflammatory challenge. Overall, these data suggest that while diets supplemented with WGP may suppress steady-state low levels of inflammatory signaling, such a supplementation may not alleviate exogenously induced massive NF-κB activation. |
topic |
Whole grape powder Inflammation NF-kB Diet In vivo Mouse |
url |
http://www.sciencedirect.com/science/article/pii/S2352385917302542 |
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