A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Brazilian Society of Chemical Engineering
1997-01-01
|
| Series: | Brazilian Journal of Chemical Engineering |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000200011 |
| Summary: | Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to achieve a high cell concentration <FONT FACE="Symbol">¾</font> 92 g l-1 dry cell weight <FONT FACE="Symbol">¾</font> and a high cell productivity <FONT FACE="Symbol">¾</font> 3.7 g l-1 h-1. |
|---|---|
| ISSN: | 0104-6632 1678-4383 |