A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION

A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION

Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to...

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Bibliographic Details
Main Authors: Gombert A.K., Kilikian B.V.
Format: Article
Language:English
Published: Brazilian Society of Chemical Engineering 1997-01-01
Series:Brazilian Journal of Chemical Engineering
Subjects:
Acetic acid
feed-forward
high cell concentration
recombinant Escherichia coli
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000200011
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spelling doaj-3fbc5a9c3a9d41c8b65f84e797a882892020-11-25T00:34:32ZengBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering0104-66321678-43831997-01-01142A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATIONGombert A.K.Kilikian B.V.Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to achieve a high cell concentration <FONT FACE="Symbol">¾</font> 92 g l-1 dry cell weight <FONT FACE="Symbol">¾</font> and a high cell productivity <FONT FACE="Symbol">¾</font> 3.7 g l-1 h-1.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000200011Acetic acidfeed-forwardhigh cell concentrationrecombinant Escherichia coli
collection DOAJ
language English
format Article
sources DOAJ
author Gombert A.K.
Kilikian B.V.
spellingShingle Gombert A.K.
Kilikian B.V.
A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
Brazilian Journal of Chemical Engineering
Acetic acid
feed-forward
high cell concentration
recombinant Escherichia coli
author_facet Gombert A.K.
Kilikian B.V.
author_sort Gombert A.K.
title A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
title_short A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
title_full A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
title_fullStr A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
title_full_unstemmed A SIMPLE WAY OF ACHIEVING A HIGH CELL CONCENTRATION IN RECOMBINANT Escherichia coli CULTIVATION
title_sort simple way of achieving a high cell concentration in recombinant escherichia coli cultivation
publisher Brazilian Society of Chemical Engineering
series Brazilian Journal of Chemical Engineering
issn 0104-6632
1678-4383
publishDate 1997-01-01
description Abstract - A cultivation strategy based on some previous knowledge of the metabolism of Escherichia coli BL21 (DE3) pLysS containing the troponin C gene cloned into plasmid pET was developed and applied through the use of simple fermentation equipment and a feed-forward control strategy in order to achieve a high cell concentration <FONT FACE="Symbol">¾</font> 92 g l-1 dry cell weight <FONT FACE="Symbol">¾</font> and a high cell productivity <FONT FACE="Symbol">¾</font> 3.7 g l-1 h-1.
topic Acetic acid
feed-forward
high cell concentration
recombinant Escherichia coli
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000200011
work_keys_str_mv AT gombertak asimplewayofachievingahighcellconcentrationinrecombinantescherichiacolicultivation
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