Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation
Reactive oxygen species (ROS) are involved in the pathologic process of pulmonary arterial hypertension as either mediators or inducers. Rutin is a type of flavonoid which exhibits significant scavenging properties on oxygen radicals both in vitro and in vivo. In this study, we proposed that rutin a...
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doaj-3fbcbc65745d405dbe8f6161b0b7bba82020-11-24T23:15:18ZengMDPI AGMolecules1420-30492014-11-011911190361904910.3390/molecules191119036molecules191119036Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell ProliferationQian Li0Yanli Qiu1Min Mao2Jinying Lv3Lixin Zhang4Shuzhen Li5Xia Li6Xiaodong Zheng7Department of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Nangang District, Harbin 150081, ChinaDepartment of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Nangang District, Harbin 150081, ChinaBio-pharmaceutical Key Laboratory of Harbin, Harbin Medical University, Harbin 150081, ChinaDepartment of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Nangang District, Harbin 150081, ChinaBio-pharmaceutical Key Laboratory of Harbin, Harbin Medical University, Harbin 150081, ChinaBio-pharmaceutical Key Laboratory of Harbin, Harbin Medical University, Harbin 150081, ChinaDepartment of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Nangang District, Harbin 150081, ChinaDepartment of Pathophysiology, Harbin Medical University-Daqing, Daqing 163319, ChinaReactive oxygen species (ROS) are involved in the pathologic process of pulmonary arterial hypertension as either mediators or inducers. Rutin is a type of flavonoid which exhibits significant scavenging properties on oxygen radicals both in vitro and in vivo. In this study, we proposed that rutin attenuated hypoxia-induced pulmonary artery smooth muscle cell (PASMC) proliferation by scavenging ROS. Immunofluorescence data showed that rutin decreased the production of ROS, which was mainly generated through mitochondria and NADPH oxidase 4 (Nox4) in pulmonary artery endothelial cells (PAECs). Western blot results provided further evidence on rutin increasing expression of Nox4 and hypoxia-inducible factor-1α (HIF-1α). Moreover, cell cycle analysis by flow cytometry indicated that proliferation of PASMCs triggered by hypoxia was also repressed by rutin. However, N-acetyl-L-cysteine (NAC), a scavenger of ROS, abolished or diminished the capability of rutin in repressing hypoxia-induced cell proliferation. These data suggest that rutin shows a potential benefit against the development of hypoxic pulmonary arterial hypertension by inhibiting ROS, subsequently preventing hypoxia-induced PASMC proliferation.http://www.mdpi.com/1420-3049/19/11/19036rutinreactive oxygen species (ROS)NADPH oxidase 4proliferationhypoxia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qian Li Yanli Qiu Min Mao Jinying Lv Lixin Zhang Shuzhen Li Xia Li Xiaodong Zheng |
spellingShingle |
Qian Li Yanli Qiu Min Mao Jinying Lv Lixin Zhang Shuzhen Li Xia Li Xiaodong Zheng Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation Molecules rutin reactive oxygen species (ROS) NADPH oxidase 4 proliferation hypoxia |
author_facet |
Qian Li Yanli Qiu Min Mao Jinying Lv Lixin Zhang Shuzhen Li Xia Li Xiaodong Zheng |
author_sort |
Qian Li |
title |
Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation |
title_short |
Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation |
title_full |
Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation |
title_fullStr |
Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation |
title_full_unstemmed |
Antioxidant Mechanism of Rutin on Hypoxia-Induced Pulmonary Arterial Cell Proliferation |
title_sort |
antioxidant mechanism of rutin on hypoxia-induced pulmonary arterial cell proliferation |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2014-11-01 |
description |
Reactive oxygen species (ROS) are involved in the pathologic process of pulmonary arterial hypertension as either mediators or inducers. Rutin is a type of flavonoid which exhibits significant scavenging properties on oxygen radicals both in vitro and in vivo. In this study, we proposed that rutin attenuated hypoxia-induced pulmonary artery smooth muscle cell (PASMC) proliferation by scavenging ROS. Immunofluorescence data showed that rutin decreased the production of ROS, which was mainly generated through mitochondria and NADPH oxidase 4 (Nox4) in pulmonary artery endothelial cells (PAECs). Western blot results provided further evidence on rutin increasing expression of Nox4 and hypoxia-inducible factor-1α (HIF-1α). Moreover, cell cycle analysis by flow cytometry indicated that proliferation of PASMCs triggered by hypoxia was also repressed by rutin. However, N-acetyl-L-cysteine (NAC), a scavenger of ROS, abolished or diminished the capability of rutin in repressing hypoxia-induced cell proliferation. These data suggest that rutin shows a potential benefit against the development of hypoxic pulmonary arterial hypertension by inhibiting ROS, subsequently preventing hypoxia-induced PASMC proliferation. |
topic |
rutin reactive oxygen species (ROS) NADPH oxidase 4 proliferation hypoxia |
url |
http://www.mdpi.com/1420-3049/19/11/19036 |
work_keys_str_mv |
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