Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste
Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate<br />(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB is<br />potential as future bioplastic but its price is very expensive due to glucos...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2015-12-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/8634 |
| Summary: | Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate<br />(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB is<br />potential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. The<br />development of PHB production using starch as an alternative carbon source has been conducted to reduce<br />the dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processing<br />site were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of the<br />study showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB ranged<br />from 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 and<br />after optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubation<br />time. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed as<br />Micrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicated<br />as poly-β- hydroxybutyrate (PHB) |
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| ISSN: | 0853-8654 2089-2241 |