Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device
Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stack...
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Online Access: | http://dx.doi.org/10.1155/2016/6939018 |
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doaj-4001c16816b046dfb42aff699f192dde2020-11-25T01:06:28ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/69390186939018Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle DeviceWoraphong Manuskiatti0Penvadee Pattanaprichakul1Siriluk Inthasotti2Panitta Sitthinamsuwan3Suchanan Hanamornroongruang4Rungsima Wanitphakdeedecha5Sorawuth Chu-ongsakol6Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDivision of Plastic Surgery, Department of Surgery, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandBackground. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stacking of a FRMS in dark-skinned subjects. Methods. Two female volunteers who were scheduled for abdominoplasty received treatment with a FRMS with varying energy settings at 6 time periods including 3 months, 1 month, 1 week, 3 days, 1 day, and the time immediately before abdominoplasty. Biopsy specimens were analyzed using hematoxylin and eosin (H&E), Verhoeff-Van Gieson (VVG), colloidal iron, and Fontana-Masson stain. Immunohistochemical study was performed by using Heat Shock Protein 70 (HSP70) antibody and collagen III monoclonal antibody. Results. The average depth of radiofrequency thermal zone (RFTZ) ranged from 100 to 300 μm, correlating with energy levels. Columns of cell necrosis and collagen denaturation followed by inflammatory response were initially demonstrated, with subsequent increasing of mucin at 1 and 3 months after treatment. Immunohistochemical study showed positive stain with HSP70. Conclusion. A single treatment with a FRMS using appropriate energy setting induces neocollagenesis. This wound healing response may serve as a mean to improve the appearance of photodamaged skin and atrophic scars.http://dx.doi.org/10.1155/2016/6939018 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Woraphong Manuskiatti Penvadee Pattanaprichakul Siriluk Inthasotti Panitta Sitthinamsuwan Suchanan Hanamornroongruang Rungsima Wanitphakdeedecha Sorawuth Chu-ongsakol |
spellingShingle |
Woraphong Manuskiatti Penvadee Pattanaprichakul Siriluk Inthasotti Panitta Sitthinamsuwan Suchanan Hanamornroongruang Rungsima Wanitphakdeedecha Sorawuth Chu-ongsakol Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device BioMed Research International |
author_facet |
Woraphong Manuskiatti Penvadee Pattanaprichakul Siriluk Inthasotti Panitta Sitthinamsuwan Suchanan Hanamornroongruang Rungsima Wanitphakdeedecha Sorawuth Chu-ongsakol |
author_sort |
Woraphong Manuskiatti |
title |
Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_short |
Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_full |
Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_fullStr |
Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_full_unstemmed |
Thermal Response of In Vivo Human Skin to Fractional Radiofrequency Microneedle Device |
title_sort |
thermal response of in vivo human skin to fractional radiofrequency microneedle device |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Background. Fractional radiofrequency microneedle system (FRMS) is a novel fractional skin resurfacing system. Data on thermal response to this fractional resurfacing technique is limited. Objectives. To investigate histologic response of in vivo human skin to varying energy settings and pulse stacking of a FRMS in dark-skinned subjects. Methods. Two female volunteers who were scheduled for abdominoplasty received treatment with a FRMS with varying energy settings at 6 time periods including 3 months, 1 month, 1 week, 3 days, 1 day, and the time immediately before abdominoplasty. Biopsy specimens were analyzed using hematoxylin and eosin (H&E), Verhoeff-Van Gieson (VVG), colloidal iron, and Fontana-Masson stain. Immunohistochemical study was performed by using Heat Shock Protein 70 (HSP70) antibody and collagen III monoclonal antibody. Results. The average depth of radiofrequency thermal zone (RFTZ) ranged from 100 to 300 μm, correlating with energy levels. Columns of cell necrosis and collagen denaturation followed by inflammatory response were initially demonstrated, with subsequent increasing of mucin at 1 and 3 months after treatment. Immunohistochemical study showed positive stain with HSP70. Conclusion. A single treatment with a FRMS using appropriate energy setting induces neocollagenesis. This wound healing response may serve as a mean to improve the appearance of photodamaged skin and atrophic scars. |
url |
http://dx.doi.org/10.1155/2016/6939018 |
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