Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China
Abstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences...
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doaj-400ad1ffe31b4fd9a03b36ba36b0c3f22020-12-08T05:03:20ZengNature Publishing GroupScientific Reports2045-23222018-07-018111010.1038/s41598-018-28469-9Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, ChinaKeqiang Huang0Yong Zhang1Yang Song2Hui Cui3Dongmei Yan4Shuangli Zhu5Qiang Sun6Haishu Tang7Dongyan Wang8Wenbo Xu9WHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionXinjiang Uygur Autonomous Region Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionXinjiang Uygur Autonomous Region Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionWHO WPRO Regional Polio Reference Laboratory and Ministry of Health Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionAbstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4–89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3Dpol region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90.https://doi.org/10.1038/s41598-018-28469-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu |
spellingShingle |
Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China Scientific Reports |
author_facet |
Keqiang Huang Yong Zhang Yang Song Hui Cui Dongmei Yan Shuangli Zhu Qiang Sun Haishu Tang Dongyan Wang Wenbo Xu |
author_sort |
Keqiang Huang |
title |
Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China |
title_short |
Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China |
title_full |
Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China |
title_fullStr |
Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China |
title_full_unstemmed |
Antigenic characteristics and genomic analysis of novel EV-A90 enteroviruses isolated in Xinjiang, China |
title_sort |
antigenic characteristics and genomic analysis of novel ev-a90 enteroviruses isolated in xinjiang, china |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-07-01 |
description |
Abstract Enterovirus A90 (EV-A90) is a novel serotype of enterovirus A species that is rarely reported. Here, we isolated five enteroviruses from patients with acute flaccid paralysis in Hotan and Kashgar cities in Xinjiang, China that were identified as EV-A90 by molecular typing. The VP1 sequences of these Xinjiang EV-A90 strains showed 88.4–89% nucleotide sequence identity to the prototype EV-A90 strain; however, genome analysis indicated complex recombination events in P2 and P3 regions. Next, the seroprevalence of EV-A90 was examined in 49 serum specimens collected in Hotan and Kashgar, and 37.5% were EV-A90 antibody positive (>1:8), with a geometric mean titre (GMT) of 1:10.47. The low positive rate and GMT suggest a low-level EV-A90 epidemic in Xinjiang. Two of the five Xinjiang EV-A90 strains were temperature sensitive, and three were temperature resistant, and a comparative genomics analysis suggested that an amino acid substitution (H1799Y) in the 3Dpol region was related to temperature sensitivity. Although the epidemic strength is low, some EV-A90 strains were temperature resistant, which is suggestive of strong virulence and transmission capacity. This study expanded the number of EV-A90 in GenBank and provided basic data that may be useful for studying the molecular epidemiology of EV-A90. |
url |
https://doi.org/10.1038/s41598-018-28469-9 |
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