Isozyme analysis of Taenia solium isolates from Mexico and Colombia

• Main Authors: Pablo Maravilla, Aldo Valera, Valeria Souza, Mario Martinez-Gordillo, Ana Flisser
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003-12-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Taenia solium; isozymes; cysticerci; Mexico; Colombia
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800013

Mexican and Colombian Taenia solium cysticerci and some species of Taenia adults were assayed using cellulose acetate electrophoresis to distinguish between isolates. Isozyme patterns for ARK, GOT, G3PD, GPI, and MPI were identical in all cysticerci suggesting homozygotic profiles. G6PD and MDH showed different patterns between Mexican and Colombian cysticerci, suggesting regional differences. ME activity was mainly detected in the adult stage suggesting that this enzyme is active in anaerobic environment, while MDH, detected in cysticerci, could be related to an environment that contains oxygen. Finally, the species of taeniid adults analyzed showed different patterns among them.