Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer
The aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically modified by introducing sulphydryl bearing compound L-cysteine HCl. Different batches of xyloglucan-cysteine conj...
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2013-06-01
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doaj-401cba02c6724f2ab1af1169c755f6332020-11-25T01:19:44ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902013-06-0149228529210.1590/S1984-82502013000200010S1984-82502013000200010Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymerMangesh Bhalekar0Savita Sonawane1Shamkant Shimpi2All India Shri Shivaji Memorial SocietyJawaharlal Nehru Technological UniversityAPL HealthcareThe aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically modified by introducing sulphydryl bearing compound L-cysteine HCl. Different batches of xyloglucan-cysteine conjugates were prepared at varying reaction pH (2-6) and evaluated for optimum thiol incorporation, disulphide group content, swelling behavior, rheological properties and mucoadhesive properties. The obtained conjugates characterized in vitro by quantification of immobilized thiol groups; showed maximum thiol incorporation on xyloglucan (7.67 ± 0.14 %) at pH 5. The disulphide group content was found maximum (2.83 ± 0.12) at pH 6. The water uptake at end of 4 h was 5.0 for xyloglucan and was found to decrease in thiolated derivatives with increase in thiolation. Mucoadhesion studies revealed that mucoadhesion of xyloglucan-cysteine conjugate increased more than twice compared to the unmodified polymer. The viscosity of thiomer was more than that of xyloglucan because of formation of disulphide bonds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200010&lng=en&tlng=enMucoadesãoTiômeroXiloglicanoCloridrato de L-cisteína |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mangesh Bhalekar Savita Sonawane Shamkant Shimpi |
spellingShingle |
Mangesh Bhalekar Savita Sonawane Shamkant Shimpi Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer Brazilian Journal of Pharmaceutical Sciences Mucoadesão Tiômero Xiloglicano Cloridrato de L-cisteína |
author_facet |
Mangesh Bhalekar Savita Sonawane Shamkant Shimpi |
author_sort |
Mangesh Bhalekar |
title |
Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
title_short |
Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
title_full |
Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
title_fullStr |
Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
title_full_unstemmed |
Synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
title_sort |
synthesis and characterization of a cysteine xyloglucan conjugate as mucoadhesive polymer |
publisher |
Universidade de São Paulo |
series |
Brazilian Journal of Pharmaceutical Sciences |
issn |
2175-9790 |
publishDate |
2013-06-01 |
description |
The aim of this study was to improve the mucoadhesive potential of xyloglucan polymer by the covalent attachment of cysteine as thiol moiety. The parent polymer xyloglucan was chemically modified by introducing sulphydryl bearing compound L-cysteine HCl. Different batches of xyloglucan-cysteine conjugates were prepared at varying reaction pH (2-6) and evaluated for optimum thiol incorporation, disulphide group content, swelling behavior, rheological properties and mucoadhesive properties. The obtained conjugates characterized in vitro by quantification of immobilized thiol groups; showed maximum thiol incorporation on xyloglucan (7.67 ± 0.14 %) at pH 5. The disulphide group content was found maximum (2.83 ± 0.12) at pH 6. The water uptake at end of 4 h was 5.0 for xyloglucan and was found to decrease in thiolated derivatives with increase in thiolation. Mucoadhesion studies revealed that mucoadhesion of xyloglucan-cysteine conjugate increased more than twice compared to the unmodified polymer. The viscosity of thiomer was more than that of xyloglucan because of formation of disulphide bonds. |
topic |
Mucoadesão Tiômero Xiloglicano Cloridrato de L-cisteína |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200010&lng=en&tlng=en |
work_keys_str_mv |
AT mangeshbhalekar synthesisandcharacterizationofacysteinexyloglucanconjugateasmucoadhesivepolymer AT savitasonawane synthesisandcharacterizationofacysteinexyloglucanconjugateasmucoadhesivepolymer AT shamkantshimpi synthesisandcharacterizationofacysteinexyloglucanconjugateasmucoadhesivepolymer |
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1725137378006269952 |