A Protocol for a Novel Human Ex Vivo Model of Aneurysm
Summary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present...
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2020-12-01
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doaj-4043a8657b8b485d84825b4fa9e0f0522020-12-21T04:48:42ZengElsevierSTAR Protocols2666-16672020-12-0113100108A Protocol for a Novel Human Ex Vivo Model of AneurysmRosaria Bianco0Karina Di Gregoli1Massimo Caputo2Sarah J. George3Jason L. Johnson4Laboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UK; Corresponding authorLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UK; Corresponding authorSummary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.http://www.sciencedirect.com/science/article/pii/S2666166720300952 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosaria Bianco Karina Di Gregoli Massimo Caputo Sarah J. George Jason L. Johnson |
spellingShingle |
Rosaria Bianco Karina Di Gregoli Massimo Caputo Sarah J. George Jason L. Johnson A Protocol for a Novel Human Ex Vivo Model of Aneurysm STAR Protocols |
author_facet |
Rosaria Bianco Karina Di Gregoli Massimo Caputo Sarah J. George Jason L. Johnson |
author_sort |
Rosaria Bianco |
title |
A Protocol for a Novel Human Ex Vivo Model of Aneurysm |
title_short |
A Protocol for a Novel Human Ex Vivo Model of Aneurysm |
title_full |
A Protocol for a Novel Human Ex Vivo Model of Aneurysm |
title_fullStr |
A Protocol for a Novel Human Ex Vivo Model of Aneurysm |
title_full_unstemmed |
A Protocol for a Novel Human Ex Vivo Model of Aneurysm |
title_sort |
protocol for a novel human ex vivo model of aneurysm |
publisher |
Elsevier |
series |
STAR Protocols |
issn |
2666-1667 |
publishDate |
2020-12-01 |
description |
Summary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics. |
url |
http://www.sciencedirect.com/science/article/pii/S2666166720300952 |
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