A Protocol for a Novel Human Ex Vivo Model of Aneurysm

Summary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present...

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Main Authors: Rosaria Bianco, Karina Di Gregoli, Massimo Caputo, Sarah J. George, Jason L. Johnson
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300952
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spelling doaj-4043a8657b8b485d84825b4fa9e0f0522020-12-21T04:48:42ZengElsevierSTAR Protocols2666-16672020-12-0113100108A Protocol for a Novel Human Ex Vivo Model of AneurysmRosaria Bianco0Karina Di Gregoli1Massimo Caputo2Sarah J. George3Jason L. Johnson4Laboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UK; Corresponding authorLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UKLaboratory of Cardiovascular Pathology, Department of Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol, Bristol, UK; Corresponding authorSummary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.http://www.sciencedirect.com/science/article/pii/S2666166720300952
collection DOAJ
language English
format Article
sources DOAJ
author Rosaria Bianco
Karina Di Gregoli
Massimo Caputo
Sarah J. George
Jason L. Johnson
spellingShingle Rosaria Bianco
Karina Di Gregoli
Massimo Caputo
Sarah J. George
Jason L. Johnson
A Protocol for a Novel Human Ex Vivo Model of Aneurysm
STAR Protocols
author_facet Rosaria Bianco
Karina Di Gregoli
Massimo Caputo
Sarah J. George
Jason L. Johnson
author_sort Rosaria Bianco
title A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_short A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_full A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_fullStr A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_full_unstemmed A Protocol for a Novel Human Ex Vivo Model of Aneurysm
title_sort protocol for a novel human ex vivo model of aneurysm
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: Aortic aneurysm rupture is a significant cause of premature mortality worldwide. Although animal models exist, some frequently experience aortic rupture and sudden death. An alternative approach is therefore required that would use human material to aid translation. Accordingly, we present an optimized and validated protocol to isolate human umbilical cord arteries and their subsequent deployment within a bioreactor. Consequently, this reproducible ex vivo human model of aneurysm can be used for pathogenesis studies and accompanying assessment of potential novel therapeutics.
url http://www.sciencedirect.com/science/article/pii/S2666166720300952
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