Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2

Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2

Induced pluripotent stem cell lines (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a ten years old boy with the type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2. PBMCs were reprogrammed using non-i...

Full description

Bibliographic Details
Main Authors: Tao Wang, Yafei Zhou, Rui Zhou, Wenjun Huang, Jie Wang, Huan Li, Ming Lei, Xiaoqiu Tan, Yanmin Zhang
Format: Article
Language:English
Published: Elsevier 2021-10-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121003561
id doaj-4044304dd84349749be56057a4bfcd86
record_format Article
spelling doaj-4044304dd84349749be56057a4bfcd862021-08-24T04:06:52ZengElsevierStem Cell Research1873-50612021-10-0156102509Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2Tao Wang0Yafei Zhou1Rui Zhou2Wenjun Huang3Jie Wang4Huan Li5Ming Lei6Xiaoqiu Tan7Yanmin Zhang8Department of Cardiology, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, ChinaNational Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children’s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an, China; Corresponding author at: National Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children's Health and Diseases, Shaanxi Institute for Pediatric Diseases, Department of Cardiology, Xi’an Children's Hospital, No. 69, Xijuyuan Lane, Xi'an 710003, China.National Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children’s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an, ChinaNational Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children’s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an, ChinaNational Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children’s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Cardiology, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, ChinaDepartment of Pharmacology, University of Oxford, Oxford OX1 3QT, UKKey Laboratory of Medical Electrophysiology of the Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, ChinaDepartment of Cardiology, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, China; National Regional Children’s Medical Center (Northwest), Key Laboratory of Precision Medicine to Pediatric Diseases of Shaanxi Province, Xi’an Key Laboratory of Children’s Health and Diseases, Shaanxi Institute for Pediatric Diseases, Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University, Xi’an, ChinaInduced pluripotent stem cell lines (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a ten years old boy with the type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2. PBMCs were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry c.1814C>T(p.P605L) mutation in KCNH2 and have a normal karyotype. Thuse the iPSC line we established will be useful for studying the pathogenesis of the type 2 long QT syndrome and drug testing.http://www.sciencedirect.com/science/article/pii/S1873506121003561
collection DOAJ
language English
format Article
sources DOAJ
author Tao Wang
Yafei Zhou
Rui Zhou
Wenjun Huang
Jie Wang
Huan Li
Ming Lei
Xiaoqiu Tan
Yanmin Zhang
spellingShingle Tao Wang
Yafei Zhou
Rui Zhou
Wenjun Huang
Jie Wang
Huan Li
Ming Lei
Xiaoqiu Tan
Yanmin Zhang
Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
Stem Cell Research
author_facet Tao Wang
Yafei Zhou
Rui Zhou
Wenjun Huang
Jie Wang
Huan Li
Ming Lei
Xiaoqiu Tan
Yanmin Zhang
author_sort Tao Wang
title Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
title_short Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
title_full Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
title_fullStr Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
title_full_unstemmed Generation of induced pluripotent stem cells (iPSCs) from a Chinese infant (XACHi015-A) with type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2
title_sort generation of induced pluripotent stem cells (ipscs) from a chinese infant (xachi015-a) with type 2 long qt syndrome carrying the heterozygous mutation c.1814c>t(p.p605l) in kcnh2
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-10-01
description Induced pluripotent stem cell lines (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a ten years old boy with the type 2 Long QT syndrome carrying the heterozygous mutation c.1814C>T(p.P605L) in KCNH2. PBMCs were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry c.1814C>T(p.P605L) mutation in KCNH2 and have a normal karyotype. Thuse the iPSC line we established will be useful for studying the pathogenesis of the type 2 long QT syndrome and drug testing.
url http://www.sciencedirect.com/science/article/pii/S1873506121003561
work_keys_str_mv AT taowang generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT yafeizhou generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT ruizhou generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT wenjunhuang generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT jiewang generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT huanli generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT minglei generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT xiaoqiutan generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
AT yanminzhang generationofinducedpluripotentstemcellsipscsfromachineseinfantxachi015awithtype2longqtsyndromecarryingtheheterozygousmutationc1814ctpp605linkcnh2
_version_ 1721197908723761152

Similar Items