Evaluation of calcification resistance of xenopericardium treated with polyhydroxy compounds

• Main Authors: M. A. Rezvova, E. A. Ovcharenko, T. V. Glushkova, Yu. A. Kudryavtseva, L. S. Barbarash
• Format: Article
• Language: Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2021-04-01
• Series: Vestnik Transplantologii i Iskusstvennyh Organov
• Subjects: xenopericardium; polyvinyl alcohol; tannic acid; ethylene glycol diglycidyl ether; glutaraldehyde; calcification
• Online Access: https://journal.transpl.ru/vtio/article/view/1317

Calcification of biomaterials used in prosthetic heart valves has been a challenging issue in cardiovascular surgery. The objective of this work is to compare the efficiency of polyvinyl alcohol (PVA) and tannic acid (TA) modification of xenomaterials, pre-stabilized with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (EGDE), in reducing calcification. Analysis of mechanical properties evaluated under uniaxial tension, showed a significant increase in the tensile strength of the test samples compared to the control (unmodified) samples (p < 0.05). Additional treatment of GA-fixed tissue with PVA and TA significantly reduced the amount of calcium in the samples implanted into rats for a 60-day follow-up (p < 0.05). The level of calcification of samples prestabilized with EGDE and treated with PVA and TA did not differ from the control group (p = 0.063). Cumulative analysis of the study results demonstrated that the GA-fixed biomaterial modified with PVA and TA can reduce calcium-binding activity and increase strength. This indicates the prospects for clinical application of the proposed treatment methods. This being said, the issue of long-term body response requires further study of the long-term stability of the modified biomaterial under physiologic blood flow conditions.