Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery
The formation of calixarene-based liposomes was investigated, and the characterization of these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the polar group pre...
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doaj-405e842011344bd4a1fa609aa58486c22021-08-26T14:13:11ZengMDPI AGPharmaceutics1999-49232021-08-01131250125010.3390/pharmaceutics13081250Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug DeliveryJosé Antonio Lebrón0Manuel López-López1Clara B. García-Calderón2Ivan V. Rosado3Fernando R. Balestra4Pablo Huertas5Roman V. Rodik6Vitaly I. Kalchenko7Eva Bernal8María Luisa Moyá9Pilar López-Cornejo10Francisco J. Ostos11Department of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, SpainDepartment of Chemical Engineering, Physical Chemistry and Materials Science, Faculty of Experimental Sciences, University of Huelva, Campus de El Carmen, Avda. de las Fuerzas Armadas s/n, 21071 Huelva, SpainInstitute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Avda. Manuel Siurot s/n, 41013 Seville, SpainDepartment of Genetics, Faculty of Biology, University of Seville, C/Profesor García González 1, 41012 Seville, SpainDepartment of Genetics, Faculty of Biology, University of Seville, C/Profesor García González 1, 41012 Seville, SpainInstitute of Organic Chemistry, National Academy of Science of Ukraine, Murmanska Str. 5, 02660 Kiev, UkraineInstitute of Organic Chemistry, National Academy of Science of Ukraine, Murmanska Str. 5, 02660 Kiev, UkraineDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, SpainDepartment of Physical Chemistry, Faculty of Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, SpainThe formation of calixarene-based liposomes was investigated, and the characterization of these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the polar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formed with one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the results obtained, the liposomes formed with the least cytotoxic calixarene, (TEAC<sub>12</sub>)<sub>4</sub>, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that (TEAC<sub>12</sub>)<sub>4</sub>/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material, although the transfection efficiency substantially increases in the presence of an additional amount of DOPE as coadjuvant. On the other hand, the (TEAC<sub>12</sub>)<sub>4</sub>/DOPE liposomes present a high doxorubicin encapsulation efficiency, and a slow controlled release, which could diminish the side effects of the drug.https://www.mdpi.com/1999-4923/13/8/1250cationic calix[4]arenesliposomesnucleic acidstransfection efficiencydoxorubicinencapsulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Antonio Lebrón Manuel López-López Clara B. García-Calderón Ivan V. Rosado Fernando R. Balestra Pablo Huertas Roman V. Rodik Vitaly I. Kalchenko Eva Bernal María Luisa Moyá Pilar López-Cornejo Francisco J. Ostos |
spellingShingle |
José Antonio Lebrón Manuel López-López Clara B. García-Calderón Ivan V. Rosado Fernando R. Balestra Pablo Huertas Roman V. Rodik Vitaly I. Kalchenko Eva Bernal María Luisa Moyá Pilar López-Cornejo Francisco J. Ostos Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery Pharmaceutics cationic calix[4]arenes liposomes nucleic acids transfection efficiency doxorubicin encapsulation |
author_facet |
José Antonio Lebrón Manuel López-López Clara B. García-Calderón Ivan V. Rosado Fernando R. Balestra Pablo Huertas Roman V. Rodik Vitaly I. Kalchenko Eva Bernal María Luisa Moyá Pilar López-Cornejo Francisco J. Ostos |
author_sort |
José Antonio Lebrón |
title |
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery |
title_short |
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery |
title_full |
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery |
title_fullStr |
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery |
title_full_unstemmed |
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery |
title_sort |
multivalent calixarene-based liposomes as platforms for gene and drug delivery |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2021-08-01 |
description |
The formation of calixarene-based liposomes was investigated, and the characterization of these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the polar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formed with one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the results obtained, the liposomes formed with the least cytotoxic calixarene, (TEAC<sub>12</sub>)<sub>4</sub>, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that (TEAC<sub>12</sub>)<sub>4</sub>/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material, although the transfection efficiency substantially increases in the presence of an additional amount of DOPE as coadjuvant. On the other hand, the (TEAC<sub>12</sub>)<sub>4</sub>/DOPE liposomes present a high doxorubicin encapsulation efficiency, and a slow controlled release, which could diminish the side effects of the drug. |
topic |
cationic calix[4]arenes liposomes nucleic acids transfection efficiency doxorubicin encapsulation |
url |
https://www.mdpi.com/1999-4923/13/8/1250 |
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