Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles
Objective: The objectives of this study were to determine the biofilm-forming capability and anti¬microbial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for...
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doaj-406f6353c8d34bc68743f052af76ba852020-11-25T02:49:29ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102020-09-017344245110.5455/javar.2020.g440100202Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profilesIsmail Abd Elhafez Radwan Raheel0Walid Hamdy Hassan1Shaaban Salem Radwan Salem2Hala Sayed Hassan Salam3Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.Objective: The objectives of this study were to determine the biofilm-forming capability and anti¬microbial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethox¬azole-trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all iso¬lates harbored blaTEM, sul1, tetA, qnrS, blaCTX-M, and fimH with a percentage of 100%, papC (40%), and hlyA (10%). FimH was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli asso¬ciated with bovine endometritis. The detection of the fimH gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli. [J Adv Vet Anim Res 2020; 7(3.000): 442-451]http://www.ejmanager.com/fulltextpdf.php?mno=100202biofilm e. coli; endometritis; resistance; virulence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ismail Abd Elhafez Radwan Raheel Walid Hamdy Hassan Shaaban Salem Radwan Salem Hala Sayed Hassan Salam |
spellingShingle |
Ismail Abd Elhafez Radwan Raheel Walid Hamdy Hassan Shaaban Salem Radwan Salem Hala Sayed Hassan Salam Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles Journal of Advanced Veterinary and Animal Research biofilm e. coli; endometritis; resistance; virulence |
author_facet |
Ismail Abd Elhafez Radwan Raheel Walid Hamdy Hassan Shaaban Salem Radwan Salem Hala Sayed Hassan Salam |
author_sort |
Ismail Abd Elhafez Radwan Raheel |
title |
Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
title_short |
Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
title_full |
Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
title_fullStr |
Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
title_full_unstemmed |
Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
title_sort |
biofilm forming potentiality of escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles |
publisher |
Network for the Veterinarians of Bangladesh |
series |
Journal of Advanced Veterinary and Animal Research |
issn |
2311-7710 |
publishDate |
2020-09-01 |
description |
Objective: The objectives of this study were to determine the biofilm-forming capability and anti¬microbial susceptibility of Escherichia coli recovered from bovine endometritis samples.
Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes.
Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethox¬azole-trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all iso¬lates harbored blaTEM, sul1, tetA, qnrS, blaCTX-M, and fimH with a percentage of 100%, papC (40%), and hlyA (10%). FimH was the most prevalent biofilm-associated gene.
Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli asso¬ciated with bovine endometritis. The detection of the fimH gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli. [J Adv Vet Anim Res 2020; 7(3.000): 442-451] |
topic |
biofilm e. coli; endometritis; resistance; virulence |
url |
http://www.ejmanager.com/fulltextpdf.php?mno=100202 |
work_keys_str_mv |
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