Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli
Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the constructi...
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doaj-40798ec64c204da89261635aaa82c4c22021-05-05T21:37:51ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.59882Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coliAvi I Flamholz0https://orcid.org/0000-0002-9278-5479Eli Dugan1https://orcid.org/0000-0003-2400-5511Cecilia Blikstad2https://orcid.org/0000-0001-5740-926XShmuel Gleizer3Roee Ben-Nissan4Shira Amram5Niv Antonovsky6Sumedha Ravishankar7https://orcid.org/0000-0002-4026-0742Elad Noor8https://orcid.org/0000-0001-8776-4799Arren Bar-Even9https://orcid.org/0000-0002-1039-4328Ron Milo10https://orcid.org/0000-0003-1641-2299David F Savage11https://orcid.org/0000-0003-0042-2257Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelMax Planck Institute of Molecular Plant Physiology, Potsdam, GermanyDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesMany photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an Escherichia coli strain engineered to depend on rubisco carboxylation for growth. Expression of 20 CCM genes enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth in ambient air depending on the components of the CCM. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria. Reconstitution enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms.https://elifesciences.org/articles/59882co2 fixationco2 concentrating mechanismphotosynthesiscarboxysomesynthetic biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Avi I Flamholz Eli Dugan Cecilia Blikstad Shmuel Gleizer Roee Ben-Nissan Shira Amram Niv Antonovsky Sumedha Ravishankar Elad Noor Arren Bar-Even Ron Milo David F Savage |
spellingShingle |
Avi I Flamholz Eli Dugan Cecilia Blikstad Shmuel Gleizer Roee Ben-Nissan Shira Amram Niv Antonovsky Sumedha Ravishankar Elad Noor Arren Bar-Even Ron Milo David F Savage Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli eLife co2 fixation co2 concentrating mechanism photosynthesis carboxysome synthetic biology |
author_facet |
Avi I Flamholz Eli Dugan Cecilia Blikstad Shmuel Gleizer Roee Ben-Nissan Shira Amram Niv Antonovsky Sumedha Ravishankar Elad Noor Arren Bar-Even Ron Milo David F Savage |
author_sort |
Avi I Flamholz |
title |
Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli |
title_short |
Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli |
title_full |
Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli |
title_fullStr |
Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli |
title_full_unstemmed |
Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli |
title_sort |
functional reconstitution of a bacterial co2 concentrating mechanism in escherichia coli |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-10-01 |
description |
Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an Escherichia coli strain engineered to depend on rubisco carboxylation for growth. Expression of 20 CCM genes enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth in ambient air depending on the components of the CCM. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria. Reconstitution enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms. |
topic |
co2 fixation co2 concentrating mechanism photosynthesis carboxysome synthetic biology |
url |
https://elifesciences.org/articles/59882 |
work_keys_str_mv |
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