Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region
Major histocompatibility complex class I chain-related A (MICA) is a highly polymorphic gene located within the MHC class I region of the human genome. Expressed as a cell surface glycoprotein, MICA modulates immune surveillance by binding to its cognate receptor on natural killer cells, NKG2D, and...
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Associação Brasileira de Divulgação Científica
2012-03-01
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doaj-40c0faf169894214ac16aa170bb5e8f82020-11-25T01:30:17ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2012-03-01453222229Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake regionZheng GongQi-Zhi LuoLin LinYu-Ping SuHai-Bo PengKun DuPing YuShi-Ping WangMajor histocompatibility complex class I chain-related A (MICA) is a highly polymorphic gene located within the MHC class I region of the human genome. Expressed as a cell surface glycoprotein, MICA modulates immune surveillance by binding to its cognate receptor on natural killer cells, NKG2D, and its genetic polymorphisms have been recently associated with susceptibility to some infectious diseases. We determined whether MICA polymorphisms were associated with the high rate of Schistosoma parasitic worm infection or severity of disease outcome in the Dongting Lake region of Hunan Province, China. Polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (SBT) were applied for high-resolution allele typing of schistosomiasis cases (N = 103, age range = 36.2-80.5 years, 64 males and 39 females) and healthy controls (N = 141, age range = 28.6-73.3 years, 73 males and 68 females). Fourteen MICA alleles and five short-tandem repeat (STR) alleles were identified among the two populations. Three (MICA*012:01/02, MICA*017 and MICA*027) showed a higher frequency in healthy controls than in schistosomiasis patients, but the difference was not significantly correlated with susceptibility to S. japonicum infection (Pc > 0.05). In contrast, higher MICA*A5 allele frequency was significantly correlated with advanced liver fibrosis (Pc < 0.05). Furthermore, the distribution profile of MICA alleles in this Hunan Han population was significantly different from those published for Korean, Thai, American-Caucasian, and Afro-American populations (P < 0.01), but similar to other Han populations within China (P > 0.05). This study provides the initial evidence that MICA genetic polymorphisms may underlie the severity of liver fibrosis occurring in schistosomiasis patients from the Dongting Lake region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300007Schistosoma japonicumMICANKG2DGene polymorphismLiver fibrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zheng Gong Qi-Zhi Luo Lin Lin Yu-Ping Su Hai-Bo Peng Kun Du Ping Yu Shi-Ping Wang |
spellingShingle |
Zheng Gong Qi-Zhi Luo Lin Lin Yu-Ping Su Hai-Bo Peng Kun Du Ping Yu Shi-Ping Wang Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region Brazilian Journal of Medical and Biological Research Schistosoma japonicum MICA NKG2D Gene polymorphism Liver fibrosis |
author_facet |
Zheng Gong Qi-Zhi Luo Lin Lin Yu-Ping Su Hai-Bo Peng Kun Du Ping Yu Shi-Ping Wang |
author_sort |
Zheng Gong |
title |
Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region |
title_short |
Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region |
title_full |
Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region |
title_fullStr |
Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region |
title_full_unstemmed |
Association of MICA gene polymorphisms with liver fibrosis in schistosomiasis patients in the Dongting Lake region |
title_sort |
association of mica gene polymorphisms with liver fibrosis in schistosomiasis patients in the dongting lake region |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2012-03-01 |
description |
Major histocompatibility complex class I chain-related A (MICA) is a highly polymorphic gene located within the MHC class I region of the human genome. Expressed as a cell surface glycoprotein, MICA modulates immune surveillance by binding to its cognate receptor on natural killer cells, NKG2D, and its genetic polymorphisms have been recently associated with susceptibility to some infectious diseases. We determined whether MICA polymorphisms were associated with the high rate of Schistosoma parasitic worm infection or severity of disease outcome in the Dongting Lake region of Hunan Province, China. Polymerase chain reaction-sequence specific priming (PCR-SSP) and sequencing-based typing (SBT) were applied for high-resolution allele typing of schistosomiasis cases (N = 103, age range = 36.2-80.5 years, 64 males and 39 females) and healthy controls (N = 141, age range = 28.6-73.3 years, 73 males and 68 females). Fourteen MICA alleles and five short-tandem repeat (STR) alleles were identified among the two populations. Three (MICA*012:01/02, MICA*017 and MICA*027) showed a higher frequency in healthy controls than in schistosomiasis patients, but the difference was not significantly correlated with susceptibility to S. japonicum infection (Pc > 0.05). In contrast, higher MICA*A5 allele frequency was significantly correlated with advanced liver fibrosis (Pc < 0.05). Furthermore, the distribution profile of MICA alleles in this Hunan Han population was significantly different from those published for Korean, Thai, American-Caucasian, and Afro-American populations (P < 0.01), but similar to other Han populations within China (P > 0.05). This study provides the initial evidence that MICA genetic polymorphisms may underlie the severity of liver fibrosis occurring in schistosomiasis patients from the Dongting Lake region. |
topic |
Schistosoma japonicum MICA NKG2D Gene polymorphism Liver fibrosis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300007 |
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