ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Amino acids are the building blocks of proteins and are considered as the key precursors for the formation of hormones and low molecular weight nitrogenous substances with biological importance. Since the analysis of amino acids has been carried out for both qualitative and quantitative purposes wit...
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doaj-40d7f2461deb4be48360e3d141b684312020-11-24T23:34:31ZengBaqai University PressBaqai Journal of Health Sciences2312-44232312-68842016-06-011915157ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHYQurat E Noor Baig0Raheela Bano1Adeel Arsalan2Zubair Anwar3Iqbal Ahmad4Department of Pharmacology, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, PakistanDepartment of Pharmaceutics, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, PakistanDepartment of Pharmaceutics, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, PakistanDepartment of Pharmaceutical Chemistry, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, PakistanDepartment of Pharmaceutical Chemistry, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, PakistanAmino acids are the building blocks of proteins and are considered as the key precursors for the formation of hormones and low molecular weight nitrogenous substances with biological importance. Since the analysis of amino acids has been carried out for both qualitative and quantitative purposes with an aim to study their levels in the plasma concentration, the quantitative determination, in particular, also helps in the diagnosis of different diseases associated with their deficiency. This review article deals with the determination of amino acids by chromatographic methods which include ion-exchange chromatography (IEC), high performance liquid chromatography (HPLC), reverse phase-high performance liquid chromatography (RP-HPLC) and ultra-performance liquid chromatography (UPLC). The review will also give an idea for the preparation of samples, derivatization methods for the analysis of amino acids (direct and indirect methods) and separation of amino acids by high performance liquid chromatographic technique.http://www.baqaiassets.com/bjhs//admin/articlefiles/1467276940upl3.pdfHPLCamino acidsderivatzationplasma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qurat E Noor Baig Raheela Bano Adeel Arsalan Zubair Anwar Iqbal Ahmad |
spellingShingle |
Qurat E Noor Baig Raheela Bano Adeel Arsalan Zubair Anwar Iqbal Ahmad ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Baqai Journal of Health Sciences HPLC amino acids derivatzation plasma |
author_facet |
Qurat E Noor Baig Raheela Bano Adeel Arsalan Zubair Anwar Iqbal Ahmad |
author_sort |
Qurat E Noor Baig |
title |
ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
title_short |
ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
title_full |
ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
title_fullStr |
ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
title_full_unstemmed |
ANALYSIS OF AMINO ACIDS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
title_sort |
analysis of amino acids by high performance liquid chromatography |
publisher |
Baqai University Press |
series |
Baqai Journal of Health Sciences |
issn |
2312-4423 2312-6884 |
publishDate |
2016-06-01 |
description |
Amino acids are the building blocks of proteins and are considered as the key precursors for the formation of hormones and low molecular weight nitrogenous substances with biological importance. Since the analysis of amino acids has been carried out for both qualitative and quantitative purposes with an aim to study their levels in the plasma concentration, the quantitative determination, in particular, also helps in the diagnosis of different diseases associated with their deficiency. This review article deals with the determination of amino acids by chromatographic methods which include ion-exchange chromatography (IEC), high performance liquid chromatography (HPLC), reverse phase-high performance liquid chromatography (RP-HPLC) and ultra-performance liquid chromatography (UPLC). The review will also give an idea for the preparation of samples, derivatization methods for the analysis of amino acids (direct and indirect methods) and separation of amino acids by high performance liquid chromatographic technique. |
topic |
HPLC amino acids derivatzation plasma |
url |
http://www.baqaiassets.com/bjhs//admin/articlefiles/1467276940upl3.pdf |
work_keys_str_mv |
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