Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress
The present study was launched to assess the effect of extreme ambient temperature associated stress on fuelling of TCA cycle in hepatic cells of Marwari goat. Based on the fact that whenever a hepatocyte needs fuel for TCA cycle, the activity of enzyme glutamate dehydrogenase (GD) increases making...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
"Vikol publishing" ST Kolesnichenko V.V.
2010-11-01
|
Series: | Journal of Stress Physiology & Biochemistry |
Subjects: | |
Online Access: | http://www.jspb.ru/issues/2010/N4/JSPB_2010_4_05-11.pdf |
id |
doaj-40df9e14dad64a419838399f2d850307 |
---|---|
record_format |
Article |
spelling |
doaj-40df9e14dad64a419838399f2d8503072020-11-24T23:16:12Zeng"Vikol publishing" ST Kolesnichenko V.V. Journal of Stress Physiology & Biochemistry1997-08382010-11-0164511Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stressKataria N.Kataria A.K.Joshi A.The present study was launched to assess the effect of extreme ambient temperature associated stress on fuelling of TCA cycle in hepatic cells of Marwari goat. Based on the fact that whenever a hepatocyte needs fuel for TCA cycle, the activity of enzyme glutamate dehydrogenase (GD) increases making alpha-ketoglutarate available for TCA cycle, 600 apparently healthy Marwari goats of either sex, between 6 months to 3 years of age were screened and blood samples were collected during moderate, cold and hot ambient temperature periods to determine the serum glutamate dehydrogenase enzyme and glucose concentration. The mean value of serum GD was significantly (p≤0.05) higher during cold and hot ambient temperature periods in comparison to overall moderate mean value. However, the rise was greater in cold (2.20 times) than hot ambient temperature (1.19 times). The serum GD activity was higher in male and younger animals. Serum glucose concentration showed a reverse trend as compared to serum GD activity. The results indicated that in cold condition associated stress the fuelling to TCA cycle was more than moderate and hot ambient temperature periods. Serum GD activity was also found related with glucose homeostasis. Further the study has shown that variations in the enzyme levels are not always pathological and while interpreting clinical data, a clinician must consider these variations. http://www.jspb.ru/issues/2010/N4/JSPB_2010_4_05-11.pdfAmbient temperaturecoldglucoseglutamate DehydrogenasehotMarwari goat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kataria N. Kataria A.K. Joshi A. |
spellingShingle |
Kataria N. Kataria A.K. Joshi A. Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress Journal of Stress Physiology & Biochemistry Ambient temperature cold glucose glutamate Dehydrogenase hot Marwari goat |
author_facet |
Kataria N. Kataria A.K. Joshi A. |
author_sort |
Kataria N. |
title |
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress |
title_short |
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress |
title_full |
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress |
title_fullStr |
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress |
title_full_unstemmed |
Fuelling of TCA cycle in hepatic cells Marwari goat during ambient temperature associated stress |
title_sort |
fuelling of tca cycle in hepatic cells marwari goat during ambient temperature associated stress |
publisher |
"Vikol publishing" ST Kolesnichenko V.V. |
series |
Journal of Stress Physiology & Biochemistry |
issn |
1997-0838 |
publishDate |
2010-11-01 |
description |
The present study was launched to assess the effect of extreme ambient temperature associated stress on fuelling of TCA cycle in hepatic cells of Marwari goat. Based on the fact that whenever a hepatocyte needs fuel for TCA cycle, the activity of enzyme glutamate dehydrogenase (GD) increases making alpha-ketoglutarate available for TCA cycle, 600 apparently healthy Marwari goats of either sex, between 6 months to 3 years of age were screened and blood samples were collected during moderate, cold and hot ambient temperature periods to determine the serum glutamate dehydrogenase enzyme and glucose concentration. The mean value of serum GD was significantly (p≤0.05) higher during cold and hot ambient temperature periods in comparison to overall moderate mean value. However, the rise was greater in cold (2.20 times) than hot ambient temperature (1.19 times). The serum GD activity was higher in male and younger animals. Serum glucose concentration showed a reverse trend as compared to serum GD activity. The results indicated that in cold condition associated stress the fuelling to TCA cycle was more than moderate and hot ambient temperature periods. Serum GD activity was also found related with glucose homeostasis. Further the study has shown that variations in the enzyme levels are not always pathological and while interpreting clinical data, a clinician must consider these variations. |
topic |
Ambient temperature cold glucose glutamate Dehydrogenase hot Marwari goat |
url |
http://www.jspb.ru/issues/2010/N4/JSPB_2010_4_05-11.pdf |
work_keys_str_mv |
AT katarian fuellingoftcacycleinhepaticcellsmarwarigoatduringambienttemperatureassociatedstress AT katariaak fuellingoftcacycleinhepaticcellsmarwarigoatduringambienttemperatureassociatedstress AT joshia fuellingoftcacycleinhepaticcellsmarwarigoatduringambienttemperatureassociatedstress |
_version_ |
1725588417460305920 |