Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
Abstract Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called “asymptomatic” ind...
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doaj-40e8105f917240c5a0860972afbed60b2020-11-25T03:34:50ZengBMCMalaria Journal1475-28752019-10-0118111010.1186/s12936-019-2979-4Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for eliminationAbu Naser Mohon0Sisay Getie1Nusrat Jahan2Mohammad Shafiul Alam3Dylan R. Pillai4Department of Microbiology and Infectious Disease, Cumming School of Medicine, University of CalgaryDepartment of Medical Parasitology, School of Biomedical and Laboratory Science, College of Medicine and Health Sciences, University of GondarEmerging Infections and Parasitology Laboratory, International Center for Diarrheal Disease Research, Bangladesh (icddr,b)Emerging Infections and Parasitology Laboratory, International Center for Diarrheal Disease Research, Bangladesh (icddr,b)Department of Microbiology and Infectious Disease, Cumming School of Medicine, University of CalgaryAbstract Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called “asymptomatic” individuals. Methods The development of the first ultrasensitive loop mediated amplification method capable of detecting malaria from both whole blood and dried blood spots (DBS) is described. The 18S rRNA and corresponding genes that remain stable on DBS for up to 5 months are targeted. Results In the case of Plasmodium falciparum, lower limits of detection of 25 parasite/mL and 50–100 parasite/mL from whole blood and DBS were obtained, respectively. A sensitivity of 97.0% (95% CI 82.5–99.8) and specificity of 99.1% (95% CI 97.6–99.7) was obtained for the detection of all species in asymptomatic individuals from Africa and Asia (n = 494). Conclusion This tool is ideally suited for low middle-income countries where malaria is endemic and ultrasensitive surveillance of malaria is highly desirable for elimination.http://link.springer.com/article/10.1186/s12936-019-2979-4MalariaDiagnosticsLAMPUltrasensitiveEliminationMethod |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abu Naser Mohon Sisay Getie Nusrat Jahan Mohammad Shafiul Alam Dylan R. Pillai |
spellingShingle |
Abu Naser Mohon Sisay Getie Nusrat Jahan Mohammad Shafiul Alam Dylan R. Pillai Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination Malaria Journal Malaria Diagnostics LAMP Ultrasensitive Elimination Method |
author_facet |
Abu Naser Mohon Sisay Getie Nusrat Jahan Mohammad Shafiul Alam Dylan R. Pillai |
author_sort |
Abu Naser Mohon |
title |
Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination |
title_short |
Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination |
title_full |
Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination |
title_fullStr |
Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination |
title_full_unstemmed |
Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination |
title_sort |
ultrasensitive loop mediated isothermal amplification (us-lamp) to detect malaria for elimination |
publisher |
BMC |
series |
Malaria Journal |
issn |
1475-2875 |
publishDate |
2019-10-01 |
description |
Abstract Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests. This is particularly true in surveillance of malaria at the population level that includes so-called “asymptomatic” individuals. Methods The development of the first ultrasensitive loop mediated amplification method capable of detecting malaria from both whole blood and dried blood spots (DBS) is described. The 18S rRNA and corresponding genes that remain stable on DBS for up to 5 months are targeted. Results In the case of Plasmodium falciparum, lower limits of detection of 25 parasite/mL and 50–100 parasite/mL from whole blood and DBS were obtained, respectively. A sensitivity of 97.0% (95% CI 82.5–99.8) and specificity of 99.1% (95% CI 97.6–99.7) was obtained for the detection of all species in asymptomatic individuals from Africa and Asia (n = 494). Conclusion This tool is ideally suited for low middle-income countries where malaria is endemic and ultrasensitive surveillance of malaria is highly desirable for elimination. |
topic |
Malaria Diagnostics LAMP Ultrasensitive Elimination Method |
url |
http://link.springer.com/article/10.1186/s12936-019-2979-4 |
work_keys_str_mv |
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