Bioshell Calcium Oxide (BiSCaO) Ointment for the Disinfection and Healing of <i>Pseudomonas Aeruginosa</i>-Infected Wounds in Hairless Rats

• Main Authors: Tomohiro Takayama, Masayuki Ishihara, Shingo Nakamura, Yoko Sato, Sumiyo Hiruma, Koichi Fukuda, Kaoru Murakami, Hidetaka Yokoe
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: International Journal of Molecular Sciences
• Subjects: bioshell calcium oxide (BiSCaO); bactericidal activity; ointment; infected wound; wound repair
• Online Access: https://www.mdpi.com/1422-0067/21/11/4176
• ISSN: 1661-6596; 1422-0067

Bioshell calcium oxide (BiSCaO) possesses deodorizing properties and broad microbicidal activity. This study aimed to investigate the application of BiSCaO ointment for the prevention and treatment of infection in chronic wounds in healing-impaired patients, without delaying wound healing. The bactericidal activities of 0.04, 0.2, 1, and 5 wt% BiSCaO ointment, 3 wt% povidone iodine ointment, and control (ointment only) were compared to evaluate the in vivo disinfection and healing of <i>Pseudomonas aeruginosa</i>-infected wounds in hairless rats. Treatment of the infected wounds with 0.2 wt% BiSCaO ointment daily for 3 days significantly enhanced wound healing and reduced the in vivo bacterial counts compared with povidone iodine ointment and control (no wound cleaning). Although 5 wt% BiSCaO ointment provided the lowest bacterial counts during 3 days’ treatment, it delayed wound healing. Histological examinations showed significantly advanced granulation tissue and capillary formation in wounds treated with 0.2 wt% BiSCaO ointment for 3 days compared to wounds treated with the other ointments. This study suggested that using 0.2 wt% BiSCaO ointment as a disinfectant for infected wounds and limiting disinfection to 3 days may be sufficient to avoid the negative effects of BiSCaO on wound repair.