Contraction mechanisms in composite active actin networks.
Simplified in vitro systems are ideally suited for studying the principle mechanisms of the contraction of cytoskeletal actin systems. To shed light on the dependence of the contraction mechanism on the nature of the crosslinking proteins, we study reconstituted in vitro active actin networks on dif...
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doaj-414125a9119747df880b44000e907b2b2020-11-24T22:05:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e3986910.1371/journal.pone.0039869Contraction mechanisms in composite active actin networks.Simone KöhlerAndreas R BauschSimplified in vitro systems are ideally suited for studying the principle mechanisms of the contraction of cytoskeletal actin systems. To shed light on the dependence of the contraction mechanism on the nature of the crosslinking proteins, we study reconstituted in vitro active actin networks on different length scales ranging from the molecular organization to the macroscopic contraction. Distinct contraction mechanisms are observed in polar and apolar crosslinked active gels whereas composite active gels crosslinked in a polar and apolar fashion at the same time exhibit both mechanisms simultaneously. In polar active actin/fascin networks initially bundles are formed which are then rearranged. In contrast, apolar cortexillin-I crosslinked active gels are bundled only after reorganization of actin filaments by myosin-II motor filaments.http://europepmc.org/articles/PMC3388086?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simone Köhler Andreas R Bausch |
spellingShingle |
Simone Köhler Andreas R Bausch Contraction mechanisms in composite active actin networks. PLoS ONE |
author_facet |
Simone Köhler Andreas R Bausch |
author_sort |
Simone Köhler |
title |
Contraction mechanisms in composite active actin networks. |
title_short |
Contraction mechanisms in composite active actin networks. |
title_full |
Contraction mechanisms in composite active actin networks. |
title_fullStr |
Contraction mechanisms in composite active actin networks. |
title_full_unstemmed |
Contraction mechanisms in composite active actin networks. |
title_sort |
contraction mechanisms in composite active actin networks. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Simplified in vitro systems are ideally suited for studying the principle mechanisms of the contraction of cytoskeletal actin systems. To shed light on the dependence of the contraction mechanism on the nature of the crosslinking proteins, we study reconstituted in vitro active actin networks on different length scales ranging from the molecular organization to the macroscopic contraction. Distinct contraction mechanisms are observed in polar and apolar crosslinked active gels whereas composite active gels crosslinked in a polar and apolar fashion at the same time exhibit both mechanisms simultaneously. In polar active actin/fascin networks initially bundles are formed which are then rearranged. In contrast, apolar cortexillin-I crosslinked active gels are bundled only after reorganization of actin filaments by myosin-II motor filaments. |
url |
http://europepmc.org/articles/PMC3388086?pdf=render |
work_keys_str_mv |
AT simonekohler contractionmechanismsincompositeactiveactinnetworks AT andreasrbausch contractionmechanismsincompositeactiveactinnetworks |
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