Experimental study of modifying influence of nanoaquachelate gold citrate on embryotoxicity of lead acetate in rats

• Main Authors: Shatorna V.F., Garetz V.I., Biletska E.M., Onul N.М., Nefyodova O.O., Ostrovskа S.S., Stepanov S.V., Dihno N.I.
• Format: Article
• Language: English
• Published: SE "Dnipropetrovsk medical academy of Health Ministry of Ukraine" 2014-06-01
• Series: Medičnì Perspektivi
• Subjects: nanometals; gold citrate; lead acetate; embryogenesis
• Online Access: http://medpers.dsma.dp.ua/issues/2014/N2/12-17.pdf

Among modern investigations in nanobiotechnology, only a small part of them is devoted to the influence of nanometals on reproductive organs and embryogenesis. Thus, if functions of the separate trace metals are studied well enough, the physiological role of such ultramicroelements as gold is still poorly studied especially in the field of its interaction with other metals. The aim of the research was an experimental study of peculiarities of gold aquananocitrate and lead acetate interaction and their impact on rats’ embryogenesis under condition of combined administration. There were used solutions of lead acetate and citrate gold in experimental models obtained by aquananotehnology. Solutions of metals and nanometals were injected perorally once a day during 19 days of pregnancy: solution of lead acetate in the dose of 0.05 mg/kg and gold aquananocitrate solution in the dose of 1.5 mg/kg. 3 group - control group. Influence of tested substances was assessed by integral and specific indicators using physiological, morphological and quantitative analysis methods. It was established that lead acetate in the dose of 0.05 mg/kg is characterized by severe embryotoxic effect manifested by total embryonic mortality, and reducing number of live fetuses in the offspring. Combined introduction of gold aquananocitrate and lead acetate weakens embryotoxic effect of lead, manifested by increase of corpora lutea number by 30.4%, increase of offspring number by 53.3% and decrease of fetal mortality by 2.3 times.