Development of efficient callus initiation of malta (Citrus sinensis) through tissue culture

• Main Author: Fazle Azim
• Format: Article
• Language: English
• Published: IJARIT Research Foundation 2011-12-01
• Series: International Journal of Agricultural Research, Innovation and Technology
• Subjects: Malta; Citrus sinensis; In vitro; Seed shoot formation; Callus induction
• Online Access: http://www.banglajol.info/index.php/IJARIT/article/view/13935/10000

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.