Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level
Red grape Nero d’Avola cultivar grape pomace powder (GPP) was applied during fresh ovine cheese production in order to increase polyphenol content. Before cheeses were produced, the bacteria of a freeze-dried commercial starter culture were isolated and tested in vitro against GPP. Two dominant stra...
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doaj-414d14457e2640b8ad661e3e7f8236c62021-03-10T00:03:51ZengMDPI AGFermentation2311-56372021-03-017353510.3390/fermentation7010035Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale LevelPietro Barbaccia0Gabriele Busetta1Michele Matraxia2Anna Maria Sutera3Valentina Craparo4Giancarlo Moschetti5Nicola Francesca6Luca Settanni7Raimondo Gaglio8Dipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Veterinarie, University of Messina, 98168 Messina, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento Scienze Agrarie, Alimentari e Forestali, Ed. 5, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyRed grape Nero d’Avola cultivar grape pomace powder (GPP) was applied during fresh ovine cheese production in order to increase polyphenol content. Before cheeses were produced, the bacteria of a freeze-dried commercial starter culture were isolated and tested in vitro against GPP. Two dominant strains, both resistant to GPP, were identified. Thestarter culture was inoculated in pasteurized ewe’s milk and the curd was divided into two bulks, one added with 1% (<i>w</i>/<i>w</i>) GPP and another one GPP-free. GPP did not influence the starter culture development, since lactic acid bacteria (LAB) counts were 10<sup>9</sup> CFU/g in both cheeses at 30 d. To exclude the interference of indigenous LAB, the pasteurized milk was analyzed, and several colonies of presumptive LAB were isolated, purified and typed. Four strains were allotted into <i>Enterococcus</i> and <i>Lacticaseibacillus</i> genera. The direct comparison of the polymorphic profiles of cheese bacteria evidenced the dominance of the starter culture over milk LAB. The addition of GPP increased cheese total phenolic compounds by 0.42 g GAE/kg. Sensory evaluation indicated that GPP-enriched cheese was well appreciated by the judges, providing evidence that GPP is a suitable substrate to increase the availability of total phenolic content in fresh ovine cheese.https://www.mdpi.com/2311-5637/7/1/35grape pomace powdercommercial starter cultureovine cheeseindustrial applicationtotal phenolic content |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pietro Barbaccia Gabriele Busetta Michele Matraxia Anna Maria Sutera Valentina Craparo Giancarlo Moschetti Nicola Francesca Luca Settanni Raimondo Gaglio |
spellingShingle |
Pietro Barbaccia Gabriele Busetta Michele Matraxia Anna Maria Sutera Valentina Craparo Giancarlo Moschetti Nicola Francesca Luca Settanni Raimondo Gaglio Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level Fermentation grape pomace powder commercial starter culture ovine cheese industrial application total phenolic content |
author_facet |
Pietro Barbaccia Gabriele Busetta Michele Matraxia Anna Maria Sutera Valentina Craparo Giancarlo Moschetti Nicola Francesca Luca Settanni Raimondo Gaglio |
author_sort |
Pietro Barbaccia |
title |
Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level |
title_short |
Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level |
title_full |
Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level |
title_fullStr |
Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level |
title_full_unstemmed |
Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level |
title_sort |
monitoring commercial starter culture development in presence of red grape pomace powder to produce polyphenol-enriched fresh ovine cheeses at industrial scale level |
publisher |
MDPI AG |
series |
Fermentation |
issn |
2311-5637 |
publishDate |
2021-03-01 |
description |
Red grape Nero d’Avola cultivar grape pomace powder (GPP) was applied during fresh ovine cheese production in order to increase polyphenol content. Before cheeses were produced, the bacteria of a freeze-dried commercial starter culture were isolated and tested in vitro against GPP. Two dominant strains, both resistant to GPP, were identified. Thestarter culture was inoculated in pasteurized ewe’s milk and the curd was divided into two bulks, one added with 1% (<i>w</i>/<i>w</i>) GPP and another one GPP-free. GPP did not influence the starter culture development, since lactic acid bacteria (LAB) counts were 10<sup>9</sup> CFU/g in both cheeses at 30 d. To exclude the interference of indigenous LAB, the pasteurized milk was analyzed, and several colonies of presumptive LAB were isolated, purified and typed. Four strains were allotted into <i>Enterococcus</i> and <i>Lacticaseibacillus</i> genera. The direct comparison of the polymorphic profiles of cheese bacteria evidenced the dominance of the starter culture over milk LAB. The addition of GPP increased cheese total phenolic compounds by 0.42 g GAE/kg. Sensory evaluation indicated that GPP-enriched cheese was well appreciated by the judges, providing evidence that GPP is a suitable substrate to increase the availability of total phenolic content in fresh ovine cheese. |
topic |
grape pomace powder commercial starter culture ovine cheese industrial application total phenolic content |
url |
https://www.mdpi.com/2311-5637/7/1/35 |
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