How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells

Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biof...

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Main Authors: Sabrina Völkel, Sascha Hein, Nathalie Benker, Felicitas Pfeifer, Christof Lenz, Gerald Losensky
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-01-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.03056/full
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spelling doaj-41632ec959434c72be6b8b83e9b0a1772020-11-25T00:29:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03056499187How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm CellsSabrina Völkel0Sascha Hein1Nathalie Benker2Felicitas Pfeifer3Christof Lenz4Christof Lenz5Gerald Losensky6Microbiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyMicrobial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyAtmospheric Aerosol, Institute of Applied Geosciences, Technische Universität Darmstadt, Darmstadt, GermanyMicrobiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttingen, GermanyBioanalytics, Institute of Clinical Chemistry, University Medical Center Göttingen, Göttingen, GermanyMicrobiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyHalobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu2+ and Ni2+.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu2+ and Ni2+ treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for ΔOE5245F and ΔOE2816F strains which exhibited increased and decreased biofilm mass after Ni2+ exposure, respectively. Moreover, EPS obviously plays a crucial role in H. salinarum metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms.https://www.frontiersin.org/article/10.3389/fmicb.2019.03056/fullmicrobial communitiesmetal stressextracellular polymeric substancesadhesionscanning electron microscopyproteome
collection DOAJ
language English
format Article
sources DOAJ
author Sabrina Völkel
Sascha Hein
Nathalie Benker
Felicitas Pfeifer
Christof Lenz
Christof Lenz
Gerald Losensky
spellingShingle Sabrina Völkel
Sascha Hein
Nathalie Benker
Felicitas Pfeifer
Christof Lenz
Christof Lenz
Gerald Losensky
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
Frontiers in Microbiology
microbial communities
metal stress
extracellular polymeric substances
adhesion
scanning electron microscopy
proteome
author_facet Sabrina Völkel
Sascha Hein
Nathalie Benker
Felicitas Pfeifer
Christof Lenz
Christof Lenz
Gerald Losensky
author_sort Sabrina Völkel
title How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
title_short How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
title_full How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
title_fullStr How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
title_full_unstemmed How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
title_sort how to cope with heavy metal ions: cellular and proteome-level stress response to divalent copper and nickel in halobacterium salinarum r1 planktonic and biofilm cells
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-01-01
description Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu2+ and Ni2+.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu2+ and Ni2+ treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for ΔOE5245F and ΔOE2816F strains which exhibited increased and decreased biofilm mass after Ni2+ exposure, respectively. Moreover, EPS obviously plays a crucial role in H. salinarum metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms.
topic microbial communities
metal stress
extracellular polymeric substances
adhesion
scanning electron microscopy
proteome
url https://www.frontiersin.org/article/10.3389/fmicb.2019.03056/full
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