How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells
Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biof...
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doaj-41632ec959434c72be6b8b83e9b0a1772020-11-25T00:29:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03056499187How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm CellsSabrina Völkel0Sascha Hein1Nathalie Benker2Felicitas Pfeifer3Christof Lenz4Christof Lenz5Gerald Losensky6Microbiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyMicrobial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyAtmospheric Aerosol, Institute of Applied Geosciences, Technische Universität Darmstadt, Darmstadt, GermanyMicrobiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Göttingen, GermanyBioanalytics, Institute of Clinical Chemistry, University Medical Center Göttingen, Göttingen, GermanyMicrobiology and Archaea, Department of Biology, Technische Universität Darmstadt, Darmstadt, GermanyHalobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu2+ and Ni2+.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu2+ and Ni2+ treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for ΔOE5245F and ΔOE2816F strains which exhibited increased and decreased biofilm mass after Ni2+ exposure, respectively. Moreover, EPS obviously plays a crucial role in H. salinarum metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms.https://www.frontiersin.org/article/10.3389/fmicb.2019.03056/fullmicrobial communitiesmetal stressextracellular polymeric substancesadhesionscanning electron microscopyproteome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sabrina Völkel Sascha Hein Nathalie Benker Felicitas Pfeifer Christof Lenz Christof Lenz Gerald Losensky |
spellingShingle |
Sabrina Völkel Sascha Hein Nathalie Benker Felicitas Pfeifer Christof Lenz Christof Lenz Gerald Losensky How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells Frontiers in Microbiology microbial communities metal stress extracellular polymeric substances adhesion scanning electron microscopy proteome |
author_facet |
Sabrina Völkel Sascha Hein Nathalie Benker Felicitas Pfeifer Christof Lenz Christof Lenz Gerald Losensky |
author_sort |
Sabrina Völkel |
title |
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells |
title_short |
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells |
title_full |
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells |
title_fullStr |
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells |
title_full_unstemmed |
How to Cope With Heavy Metal Ions: Cellular and Proteome-Level Stress Response to Divalent Copper and Nickel in Halobacterium salinarum R1 Planktonic and Biofilm Cells |
title_sort |
how to cope with heavy metal ions: cellular and proteome-level stress response to divalent copper and nickel in halobacterium salinarum r1 planktonic and biofilm cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-01-01 |
description |
Halobacterium salinarum R1 is an extremely halophilic archaeon capable of adhesion and forming biofilms, allowing it to adjust to a range of growth conditions. We have recently shown that living in biofilms facilitates its survival under Cu2+ and Ni2+ stress, with specific rearrangements of the biofilm architecture observed following exposition. In this study, quantitative analyses were performed by SWATH mass spectrometry to determine the respective proteomes of planktonic and biofilm cells after exposition to Cu2+ and Ni2+.Quantitative data for 1180 proteins were obtained, corresponding to 46% of the predicted proteome. In planktonic cells, 234 of 1180 proteins showed significant abundance changes after metal ion treatment, of which 47% occurred in Cu2+ and Ni2+ treated samples. In biofilms, significant changes were detected for 52 proteins. Only three proteins changed under both conditions, suggesting metal-specific stress responses in biofilms. Deletion strains were generated to assess the potential role of selected target genes. Strongest effects were observed for ΔOE5245F and ΔOE2816F strains which exhibited increased and decreased biofilm mass after Ni2+ exposure, respectively. Moreover, EPS obviously plays a crucial role in H. salinarum metal ion resistance. Further efforts are required to elucidate the molecular basis and interplay of additional resistance mechanisms. |
topic |
microbial communities metal stress extracellular polymeric substances adhesion scanning electron microscopy proteome |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.03056/full |
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