BRITER: a BMP responsive osteoblast reporter cell line.
BACKGROUND: BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is th...
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2012-01-01
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doaj-416f24f4c9154ddbbe711c415a895e972020-11-25T00:55:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3713410.1371/journal.pone.0037134BRITER: a BMP responsive osteoblast reporter cell line.Prem Swaroop YadavParitosh PrasharAmitabha BandyopadhyayBACKGROUND: BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens. METHODOLOGY/PRINCIPAL FINDINGS: We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins. CONCLUSION: As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance.http://europepmc.org/articles/PMC3354957?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Prem Swaroop Yadav Paritosh Prashar Amitabha Bandyopadhyay |
spellingShingle |
Prem Swaroop Yadav Paritosh Prashar Amitabha Bandyopadhyay BRITER: a BMP responsive osteoblast reporter cell line. PLoS ONE |
author_facet |
Prem Swaroop Yadav Paritosh Prashar Amitabha Bandyopadhyay |
author_sort |
Prem Swaroop Yadav |
title |
BRITER: a BMP responsive osteoblast reporter cell line. |
title_short |
BRITER: a BMP responsive osteoblast reporter cell line. |
title_full |
BRITER: a BMP responsive osteoblast reporter cell line. |
title_fullStr |
BRITER: a BMP responsive osteoblast reporter cell line. |
title_full_unstemmed |
BRITER: a BMP responsive osteoblast reporter cell line. |
title_sort |
briter: a bmp responsive osteoblast reporter cell line. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
BACKGROUND: BMP signaling pathway is critical for vertebrate development and tissue homeostasis. High-throughput molecular genetic screening may reveal novel players regulating BMP signaling response while chemical genetic screening of BMP signaling modifiers may have clinical significance. It is therefore important to generate a cell-based tool to execute such screens. METHODOLOGY/PRINCIPAL FINDINGS: We have established a BMP responsive reporter cell line by stably integrating a BMP responsive dual luciferase reporter construct in the immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2; Bmp4 double conditional knockout mouse strain. This cell line, named BRITER (BMP Responsive Immortalized Reporter cell line), responds robustly, promptly and specifically to exogenously added BMP2 protein. The sensitivity to added BMP may be further increased by depleting the endogenous BMP2 and BMP4 proteins. CONCLUSION: As the dynamic range of the assay (for BMP responsiveness) is very high for BRITER and as it responds specifically and promptly to exogenously added BMP2 protein, BRITER may be used effectively for chemical or molecular genetic screening for BMP signaling modifiers. Identification of novel molecular players capable of influencing BMP signaling pathway may have clinical significance. |
url |
http://europepmc.org/articles/PMC3354957?pdf=render |
work_keys_str_mv |
AT premswaroopyadav briterabmpresponsiveosteoblastreportercellline AT paritoshprashar briterabmpresponsiveosteoblastreportercellline AT amitabhabandyopadhyay briterabmpresponsiveosteoblastreportercellline |
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