The inhibitory effect of the pomegranate extract (Punica granatum) towards certain species of species Lactobacillus and Clostridium isolated from human faeces

• Main Authors: Ahmed Hekmat, Omar Al - Obeidi
• Format: Article
• Language: Arabic
• Published: College of Education for Pure Sciences 2019-03-01
• Series: مجلة التربية والعلم
• Online Access: https://edusj.mosuljournals.com/article_161049_17f3a5000386f270cd5d86709d0a6cb0.pdf

This study showed isolation and diagnosis of 70 isolates of intestinal flora that isolated from feces of healthy people in Mosul city / Iraq, distributed to 40 isolates from beneficial bacteria <em>Lactobacillus</em> spp. and 30 isolates from harmful bacteria <em>Clostridium</em> spp., this include species of beneficial bacteria : <em>L. acidophilus</em>, <em>L. casei</em> subsp. <em>casei</em>, <em>L. pentosus</em>, <em>L. reuteri</em>, <em>L. ruminis</em>, <em>L. fermentum</em>,<em> L. gasseri</em>, <em>L.</em> <em>helveticus</em>, <em>L. plantarum</em>, <em>L. paracasei</em> subsp. <em>paracasei</em>, <em>L. salivarius</em>, <em>L. casei</em> subsp.<em> rhamnosus</em> and <em>L. johnsonii</em>, as well as the species of harmful bacteria included into <em>C. butyricum</em>, <em>C. difficile</em>, <em>C. cadaveris</em>, <em>C. septicum</em>, <em>C. ramosum</em>, <em>C. histolyticum</em>, <em>C. tertium</em>, <em>C. perfringes</em> and <em>C. clostridiiforme</em>, adopted cultural qualities, microscopic and biochemical tests to get the results.<br /> Inhibitory effectiveness of the variable constrations 4, 8 and 12 mg. from alcoholic (methanol) extract of peeled fruits of pomegranate (<em>Punica granatum</em>) against the isolated bacteria was studied. The method of disks in the measurement of the effectiveness was adopted. Results showed that the plant extract has a powerful effect against pathogenic bacteria represented by <em>Clostridium</em> spp. while lower activity against bacteria <em>Lactobacillus</em> spp.