Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii

Prototheca zopfii commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patie...

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Main Authors: Xuanhao Zeng, Timothy Kudinha, Fanrong Kong, Qiang-qiang Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00443/full
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spelling doaj-4175521d77054c6f986d47d445e472d32020-11-25T00:00:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-03-011010.3389/fmicb.2019.00443412811Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfiiXuanhao Zeng0Timothy Kudinha1Fanrong Kong2Qiang-qiang Zhang3Division of Mycology, Huashan Hospital, Fudan University, Shanghai, ChinaCharles Sturt University, Leeds Parade, Orange, NSW, AustraliaCentre for Infectious Diseases and Microbiology Laboratory Services, ICPMR-Pathology West, Westmead Hospital, The University of Sydney, Sydney, NSW, AustraliaDivision of Mycology, Huashan Hospital, Fudan University, Shanghai, ChinaPrototheca zopfii commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patients has a poor prognosis due to limited understanding of the pathogenesis of the disease, as most previous studies mainly focused on classification and recognition of pathogenic strains. In this study, we constructed the genome and transcriptome of two pathogenic strains and one environmental strain, by next generation sequencing methods. Based on our preliminary gene expression findings, genes in P. zopfii pathogenic strains are significantly up-regulated in metabolism in peroxisome, such as glyoxylate cycle, which may improve the organism’s resistance to the harsh environment in phagolysosome of macrophage and its ability to survive in an anaerobic environment. We also found some significant up-regulated genes, which are related to adherence and penetration in dermatophytes, and we speculate that this may enhance the virulence capacity of pathogenic strains. Finally, the genomes and transcriptomes of P. zopfii described here provide some base for further studies on the pathogenesis of this organism.https://www.frontiersin.org/article/10.3389/fmicb.2019.00443/fullPrototheca zopfiiprotothecosisgenometranscriptomevirulence
collection DOAJ
language English
format Article
sources DOAJ
author Xuanhao Zeng
Timothy Kudinha
Fanrong Kong
Qiang-qiang Zhang
spellingShingle Xuanhao Zeng
Timothy Kudinha
Fanrong Kong
Qiang-qiang Zhang
Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
Frontiers in Microbiology
Prototheca zopfii
protothecosis
genome
transcriptome
virulence
author_facet Xuanhao Zeng
Timothy Kudinha
Fanrong Kong
Qiang-qiang Zhang
author_sort Xuanhao Zeng
title Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
title_short Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
title_full Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
title_fullStr Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
title_full_unstemmed Comparative Genome and Transcriptome Study of the Gene Expression Difference Between Pathogenic and Environmental Strains of Prototheca zopfii
title_sort comparative genome and transcriptome study of the gene expression difference between pathogenic and environmental strains of prototheca zopfii
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2019-03-01
description Prototheca zopfii commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patients has a poor prognosis due to limited understanding of the pathogenesis of the disease, as most previous studies mainly focused on classification and recognition of pathogenic strains. In this study, we constructed the genome and transcriptome of two pathogenic strains and one environmental strain, by next generation sequencing methods. Based on our preliminary gene expression findings, genes in P. zopfii pathogenic strains are significantly up-regulated in metabolism in peroxisome, such as glyoxylate cycle, which may improve the organism’s resistance to the harsh environment in phagolysosome of macrophage and its ability to survive in an anaerobic environment. We also found some significant up-regulated genes, which are related to adherence and penetration in dermatophytes, and we speculate that this may enhance the virulence capacity of pathogenic strains. Finally, the genomes and transcriptomes of P. zopfii described here provide some base for further studies on the pathogenesis of this organism.
topic Prototheca zopfii
protothecosis
genome
transcriptome
virulence
url https://www.frontiersin.org/article/10.3389/fmicb.2019.00443/full
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