The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7
TRPV4 is a type of nonselective cation channel, and activation of TRPV4 in the gastrointestinal tract causes experimental colitis in mice. A previous study found that tyrosine-phosphorylated claudin-7 is increased in experimental colitis. The relationship between tyrosine-phosphorylated claudin-7 an...
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doaj-4175e703a7c041bd980fcf7810c4b4c32021-05-20T07:39:48ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-02-01122The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7Yuan-Yuan Huang0Jing Li1He-Rui Zhang2Su-Wen Bai3Hui-Yun Yang4Bing Shen5Juan Du6Xian-Ming Xia7Department of Internal Medicine, Yangpu Hospital, Tongji University, Shanghai 200090, ChinaDepartment of Gastroenterology and Hepatology, Danyang People’s Hospital, Danyang, 212300, Jiangsu, ChinaDepartment of Gastroenterology and Hepatology, the Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230001, Anhui, ChinaDepartment of Physiology, School of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui, ChinaDepartment of Gastroenterology, the Anhui Provincial Children's Hospital, Hefei 230051, Anhui, ChinaDepartment of Physiology, School of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui, ChinaDepartment of Physiology, School of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui, China; Corresponding authors at: Department of Physiology, School of Basic Medicine, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.Department of Gastroenterology and Hepatology, the Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230001, Anhui, China; Corresponding author at: Department of Gastroenterology and Hepatology, the Fourth Affiliated Hospital of Anhui Medical University, 100 Huaihai Road, Hefei, 230001, Anhui, China.TRPV4 is a type of nonselective cation channel, and activation of TRPV4 in the gastrointestinal tract causes experimental colitis in mice. A previous study found that tyrosine-phosphorylated claudin-7 is increased in experimental colitis. The relationship between tyrosine-phosphorylated claudin-7 and TRPV4 remains undefined. In the present study, we developed a claudin-7 mutant by replacing tyrosine with glutamic acid at position 210, named cld7-Y210E colonic cells. We found that activation of TRPV4 by GSK1016790A increased the permeability of control colonic cell monolayers, which was decreased by the TRPV4 antagonist HC067047. In monolayers of cld7-Y210E colonic cells, no differences in permeability were found between GSK1016790A and HC067047 treatments. GSK1016790A increased the aggregation of claudin-7 at the cell membrane in control colonic cells, and the effect was diminished by HC067047. In cld7-Y210E colonic cells, neither GSK1016790A nor HC067047 apparently changed the aggregation of claudin-7. Neither GSK1016790A nor HC067047 altered the TRPV4 protein level in vector colonic cells. In cld7-wild colonic cells, GSK1016790A did not alter the TRPV4 protein level, while HC067047 increased the TRPV4 protein level. The TRPV4 protein level was increased in cld7-Y210E colonic cells, decreased by GSK1016790A and further decreased by HC067047. Calcium influx was not significantly changed in the control colonic cells treated with GSK1016790A. However, GSK1016790A significantly increased calcium influx in cld7-Y210E colonic cells. We concluded that tyrosine-phosphorylated claudin-7 affects the TRPV4-modulated intestinal epithelial barrier, TRPV4-mediated calcium influx, and the protein expression of TRPV4 in human colonic cells. We suggest that tyrosine-phosphorylated claudin-7 affects the TRPV4-modulated intestinal epithelial barrier, which might be related to TRPV4 expression and TRPV4-mediated calcium influx.http://www.sciencedirect.com/science/article/pii/S0753332219353193Claudin-7Tyrosine phosphorylationTransient receptor potential vanilloid 4Intestinal epithelial barrier |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan-Yuan Huang Jing Li He-Rui Zhang Su-Wen Bai Hui-Yun Yang Bing Shen Juan Du Xian-Ming Xia |
spellingShingle |
Yuan-Yuan Huang Jing Li He-Rui Zhang Su-Wen Bai Hui-Yun Yang Bing Shen Juan Du Xian-Ming Xia The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 Biomedicine & Pharmacotherapy Claudin-7 Tyrosine phosphorylation Transient receptor potential vanilloid 4 Intestinal epithelial barrier |
author_facet |
Yuan-Yuan Huang Jing Li He-Rui Zhang Su-Wen Bai Hui-Yun Yang Bing Shen Juan Du Xian-Ming Xia |
author_sort |
Yuan-Yuan Huang |
title |
The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
title_short |
The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
title_full |
The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
title_fullStr |
The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
title_full_unstemmed |
The effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
title_sort |
effect of transient receptor potential vanilloid 4 on the intestinal epithelial barrier and human colonic cells was affected by tyrosine-phosphorylated claudin-7 |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-02-01 |
description |
TRPV4 is a type of nonselective cation channel, and activation of TRPV4 in the gastrointestinal tract causes experimental colitis in mice. A previous study found that tyrosine-phosphorylated claudin-7 is increased in experimental colitis. The relationship between tyrosine-phosphorylated claudin-7 and TRPV4 remains undefined. In the present study, we developed a claudin-7 mutant by replacing tyrosine with glutamic acid at position 210, named cld7-Y210E colonic cells. We found that activation of TRPV4 by GSK1016790A increased the permeability of control colonic cell monolayers, which was decreased by the TRPV4 antagonist HC067047. In monolayers of cld7-Y210E colonic cells, no differences in permeability were found between GSK1016790A and HC067047 treatments. GSK1016790A increased the aggregation of claudin-7 at the cell membrane in control colonic cells, and the effect was diminished by HC067047. In cld7-Y210E colonic cells, neither GSK1016790A nor HC067047 apparently changed the aggregation of claudin-7. Neither GSK1016790A nor HC067047 altered the TRPV4 protein level in vector colonic cells. In cld7-wild colonic cells, GSK1016790A did not alter the TRPV4 protein level, while HC067047 increased the TRPV4 protein level. The TRPV4 protein level was increased in cld7-Y210E colonic cells, decreased by GSK1016790A and further decreased by HC067047. Calcium influx was not significantly changed in the control colonic cells treated with GSK1016790A. However, GSK1016790A significantly increased calcium influx in cld7-Y210E colonic cells. We concluded that tyrosine-phosphorylated claudin-7 affects the TRPV4-modulated intestinal epithelial barrier, TRPV4-mediated calcium influx, and the protein expression of TRPV4 in human colonic cells. We suggest that tyrosine-phosphorylated claudin-7 affects the TRPV4-modulated intestinal epithelial barrier, which might be related to TRPV4 expression and TRPV4-mediated calcium influx. |
topic |
Claudin-7 Tyrosine phosphorylation Transient receptor potential vanilloid 4 Intestinal epithelial barrier |
url |
http://www.sciencedirect.com/science/article/pii/S0753332219353193 |
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