Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176
The present report is the original description of blaTEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials classified in 15 different groups by agar diffusion and establishing the phyl...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | Spanish |
Published: |
Instituto Nacional de Salud
2021-03-01
|
Series: | Revista Peruana de Medicina Experimental y Salud Pública |
Subjects: | |
Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/6727 |
id |
doaj-417e5b9f84864c449f5cae5e76d50a4b |
---|---|
record_format |
Article |
spelling |
doaj-417e5b9f84864c449f5cae5e76d50a4b2021-05-11T21:46:26ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422021-03-01381130510.17843/rpmesp.2021.381.67272460Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176Joaquim Ruiz0Maria J. Pons1Susan Mosquito2Theresa J. Ochoa3Yolanda Sáenz4Universidad Cientiíica del SurUniversidad Cientifica del SurUniversidad Peruana Cayetano HerediaUniversidad Peruana Cayetano HerediaCentro de Investigación Biomédica de La RiojaThe present report is the original description of blaTEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials classified in 15 different groups by agar diffusion and establishing the phylogenetic group, mechanisms of resistance and presence of Class 1 and 2 integrons. Integrons and β-lactam resistance genes were sequenced. The isolate, belonging to phylogenetic group A, showed the presence of resistance or diminished susceptibility to a ampicillin, amoxicillin plus clavulanic acid, nalidíxic acid, ciprofloxacin, streptomycin, kanamycin, tetracycline, trimethoprim, sulfisoxazole, cotrimoxazole, azithromycin and nitrofurantoin, carrying blaTEM, aadA1/2, aphA1, sul3, tet(A) and a Class 2 integron containing a dfrA1 gene. Quinolone resistance was related to the substitution Ser83Ala. The TEM sequencing showed the presence of the new substitution Ala222Val, which led to the description of the new β-lactamase blaTEM-176.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/6727ampicilinaamoxicilina-ácido clavulánicobeta-lactamasasresistencia a antibióticosescherichia coli enterotoxigénicaepidemiología molecularintegrones |
collection |
DOAJ |
language |
Spanish |
format |
Article |
sources |
DOAJ |
author |
Joaquim Ruiz Maria J. Pons Susan Mosquito Theresa J. Ochoa Yolanda Sáenz |
spellingShingle |
Joaquim Ruiz Maria J. Pons Susan Mosquito Theresa J. Ochoa Yolanda Sáenz Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 Revista Peruana de Medicina Experimental y Salud Pública ampicilina amoxicilina-ácido clavulánico beta-lactamasas resistencia a antibióticos escherichia coli enterotoxigénica epidemiología molecular integrones |
author_facet |
Joaquim Ruiz Maria J. Pons Susan Mosquito Theresa J. Ochoa Yolanda Sáenz |
author_sort |
Joaquim Ruiz |
title |
Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 |
title_short |
Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 |
title_full |
Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 |
title_fullStr |
Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 |
title_full_unstemmed |
Characterization of Escherichia coli D7111 producing the β-lactamase TEM-176 |
title_sort |
characterization of escherichia coli d7111 producing the β-lactamase tem-176 |
publisher |
Instituto Nacional de Salud |
series |
Revista Peruana de Medicina Experimental y Salud Pública |
issn |
1726-4634 1726-4642 |
publishDate |
2021-03-01 |
description |
The present report is the original description of blaTEM-176. The mechanisms of resistance to antimicrobial agents were determined in an enterotoxigenic Escherichia coli, determining the susceptibility to 22 antimicrobials classified in 15 different groups by agar diffusion and establishing the phylogenetic group, mechanisms of resistance and presence of Class 1 and 2 integrons. Integrons and β-lactam resistance genes were sequenced. The isolate, belonging to phylogenetic group A, showed the presence of resistance or diminished susceptibility to a ampicillin, amoxicillin plus clavulanic acid, nalidíxic acid, ciprofloxacin, streptomycin, kanamycin, tetracycline, trimethoprim, sulfisoxazole, cotrimoxazole, azithromycin and nitrofurantoin, carrying blaTEM, aadA1/2, aphA1, sul3, tet(A) and a Class 2 integron containing a dfrA1 gene. Quinolone resistance was related to the substitution Ser83Ala. The TEM sequencing showed the presence of the new substitution Ala222Val, which led to the description of the new β-lactamase blaTEM-176. |
topic |
ampicilina amoxicilina-ácido clavulánico beta-lactamasas resistencia a antibióticos escherichia coli enterotoxigénica epidemiología molecular integrones |
url |
https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/6727 |
work_keys_str_mv |
AT joaquimruiz characterizationofescherichiacolid7111producingtheblactamasetem176 AT mariajpons characterizationofescherichiacolid7111producingtheblactamasetem176 AT susanmosquito characterizationofescherichiacolid7111producingtheblactamasetem176 AT theresajochoa characterizationofescherichiacolid7111producingtheblactamasetem176 AT yolandasaenz characterizationofescherichiacolid7111producingtheblactamasetem176 |
_version_ |
1721443503037218816 |