KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL
Bran rice is a by-product of rice into rice milling process, the cellulose content of 40-60%, so the potential as a carbon source for the growth of microorganisms such as bacteria to produce enzymes particularly cellulolytic bacteria. The purpose of the study was to determine the diversity of the ch...
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Universitas Islam Negeri Malik Ibrahim
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doaj-419560738aba433b8a7e5da83e5aa06b2020-11-24T23:08:14ZindUniversitas Islam Negeri Malik IbrahimAlchemy: Journal of Chemistry2086-17102460-68712013-05-010010.18860/al.v0i0.22972075KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATULDyah Ayu Saropah0Akyunul Jannah1Anik Maunatin2Jurusan Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik Ibrahim MalangJurusan Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik Ibrahim MalangJurusan Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik Ibrahim MalangBran rice is a by-product of rice into rice milling process, the cellulose content of 40-60%, so the potential as a carbon source for the growth of microorganisms such as bacteria to produce enzymes particularly cellulolytic bacteria. The purpose of the study was to determine the diversity of the characters from the cellulolytic bacterial isolates and optimum conditions enzyme (cellulase enzymes rough) so that they can hydrolyze the cellulose to glucose with either rice bran. The characterization includes the determination of pH, temperature and time of optimum crude extract of bacterial cellulolytic enzyme cellulase, determination of Vmax and Km and molecular mass determination of cellulase. Research methods include making media, regeneration of isolates, bacterial growth curve manufacturing, production of cellulase enzymes from bacterial cellulolytic rough at the optimum conditions, the kinetics of enzymatic reaction: substrate concentration factor of the reaction rate (with variation of the concentration of 0.50%, 0.75%, 1 , 00%, 1.25% and 1.50% (w / v)) followed by calculating the Vmax and Km. The results showed that the enzyme cellulase of cellulolytic bacteria isolated from rice bran result that has optimum conditions at pH 7.5, temperature 50 ° C, 40 min incubation time to produce Vmax 0.0086 units / mL and Km 1.694%.http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/2297kinetics of enzymatic reactionselullase enzymerice bran |
collection |
DOAJ |
language |
Indonesian |
format |
Article |
sources |
DOAJ |
author |
Dyah Ayu Saropah Akyunul Jannah Anik Maunatin |
spellingShingle |
Dyah Ayu Saropah Akyunul Jannah Anik Maunatin KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL Alchemy: Journal of Chemistry kinetics of enzymatic reaction selullase enzyme rice bran |
author_facet |
Dyah Ayu Saropah Akyunul Jannah Anik Maunatin |
author_sort |
Dyah Ayu Saropah |
title |
KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL |
title_short |
KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL |
title_full |
KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL |
title_fullStr |
KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL |
title_full_unstemmed |
KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL |
title_sort |
kinetika reaksi enzimatis ekstrak kasar enzim selulase bakteri selulolitik hasil isolasi dari bekatul |
publisher |
Universitas Islam Negeri Malik Ibrahim |
series |
Alchemy: Journal of Chemistry |
issn |
2086-1710 2460-6871 |
publishDate |
2013-05-01 |
description |
Bran rice is a by-product of rice into rice milling process, the cellulose content of 40-60%, so the potential as a carbon source for the growth of microorganisms such as bacteria to produce enzymes particularly cellulolytic bacteria. The purpose of the study was to determine the diversity of the characters from the cellulolytic bacterial isolates and optimum conditions enzyme (cellulase enzymes rough) so that they can hydrolyze the cellulose to glucose with either rice bran. The characterization includes the determination of pH, temperature and time of optimum crude extract of bacterial cellulolytic enzyme cellulase, determination of Vmax and Km and molecular mass determination of cellulase.
Research methods include making media, regeneration of isolates, bacterial growth curve manufacturing, production of cellulase enzymes from bacterial cellulolytic rough at the optimum conditions, the kinetics of enzymatic reaction: substrate concentration factor of the reaction rate (with variation of the concentration of 0.50%, 0.75%, 1 , 00%, 1.25% and 1.50% (w / v)) followed by calculating the Vmax and Km.
The results showed that the enzyme cellulase of cellulolytic bacteria isolated from rice bran result that has optimum conditions at pH 7.5, temperature 50 ° C, 40 min incubation time to produce Vmax 0.0086 units / mL and Km 1.694%. |
topic |
kinetics of enzymatic reaction selullase enzyme rice bran |
url |
http://ejournal.uin-malang.ac.id/index.php/Kimia/article/view/2297 |
work_keys_str_mv |
AT dyahayusaropah kinetikareaksienzimatisekstrakkasarenzimselulasebakteriselulolitikhasilisolasidaribekatul AT akyunuljannah kinetikareaksienzimatisekstrakkasarenzimselulasebakteriselulolitikhasilisolasidaribekatul AT anikmaunatin kinetikareaksienzimatisekstrakkasarenzimselulasebakteriselulolitikhasilisolasidaribekatul |
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