Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK
Background: Dihydroartemisinin (DHA) possesses an inhibitory effect on ovarian cancer and promotes reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression in glioma cells. This study explored the role of DHA and RECK on ovarian cancer. Methods: The RECK level in ovarian cancer w...
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2021-06-01
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doaj-419c92b4b4cd45d5914fe2d1e06904642021-05-02T05:53:35ZengElsevierJournal of Pharmacological Sciences1347-86132021-06-0114627181Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECKJingfei Zheng0Xuehe Li1Weili Yang2Fang Zhang3Department of Obstetrics and Gynecology, The Affiliated People's Hospital of Ningbo University, China; Corresponding author. Department of Obstetrics and Gynecology, The Affiliated People's Hospital of Ningbo University, No. 251, Baizhang East Road, Yinzhou District, Ningbo, 315000, China.Department of Gynecology, The Affiliated People's Hospital of Ningbo University, ChinaDepartment of Gynecology, The Affiliated People's Hospital of Ningbo University, ChinaDepartment of Gynecology, The Affiliated People's Hospital of Ningbo University, ChinaBackground: Dihydroartemisinin (DHA) possesses an inhibitory effect on ovarian cancer and promotes reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression in glioma cells. This study explored the role of DHA and RECK on ovarian cancer. Methods: The RECK level in ovarian cancer was analyzed under GEPIA 2 database and proved by RT-qPCR. After being treated with DHA or infected with siRECK lentivirus, the viability, apoptosis, migration, and invasion of ovarian cancer cells were evaluated by CCK-8, flow cytometry, wound healing, and transwell assays. Also, the expressions of factors related to apoptosis and epithelial-mesenchymal transition were measured by Western blot or RT-qPCR. Results: DHA-treatment weakened the viability, migration, invasion, and enhanced apoptosis of ovarian cancer cells. DHA also down-regulated the levels of Bcl-2, N-cadherin, and Vimentin, and up-regulated the levels of Bax, C-caspase-3 and E-cadherin in ovarian cancer cells. RECK was lowly expressed in both ovarian cancer tissues and cells. siRECK not only had an effect opposite to DHA on the viability, apoptosis, migration, invasion, and related-factors of ovarian cancer cells but also offset the effect of DHA on ovarian cancer cells. Conclusion: DHA regulated apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK.http://www.sciencedirect.com/science/article/pii/S1347861321000128Ovarian cancerDihydroartemisininRECKApoptosisInvasion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jingfei Zheng Xuehe Li Weili Yang Fang Zhang |
spellingShingle |
Jingfei Zheng Xuehe Li Weili Yang Fang Zhang Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK Journal of Pharmacological Sciences Ovarian cancer Dihydroartemisinin RECK Apoptosis Invasion |
author_facet |
Jingfei Zheng Xuehe Li Weili Yang Fang Zhang |
author_sort |
Jingfei Zheng |
title |
Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK |
title_short |
Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK |
title_full |
Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK |
title_fullStr |
Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK |
title_full_unstemmed |
Dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK |
title_sort |
dihydroartemisinin regulates apoptosis, migration, and invasion of ovarian cancer cells via mediating reck |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2021-06-01 |
description |
Background: Dihydroartemisinin (DHA) possesses an inhibitory effect on ovarian cancer and promotes reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression in glioma cells. This study explored the role of DHA and RECK on ovarian cancer. Methods: The RECK level in ovarian cancer was analyzed under GEPIA 2 database and proved by RT-qPCR. After being treated with DHA or infected with siRECK lentivirus, the viability, apoptosis, migration, and invasion of ovarian cancer cells were evaluated by CCK-8, flow cytometry, wound healing, and transwell assays. Also, the expressions of factors related to apoptosis and epithelial-mesenchymal transition were measured by Western blot or RT-qPCR. Results: DHA-treatment weakened the viability, migration, invasion, and enhanced apoptosis of ovarian cancer cells. DHA also down-regulated the levels of Bcl-2, N-cadherin, and Vimentin, and up-regulated the levels of Bax, C-caspase-3 and E-cadherin in ovarian cancer cells. RECK was lowly expressed in both ovarian cancer tissues and cells. siRECK not only had an effect opposite to DHA on the viability, apoptosis, migration, invasion, and related-factors of ovarian cancer cells but also offset the effect of DHA on ovarian cancer cells. Conclusion: DHA regulated apoptosis, migration, and invasion of ovarian cancer cells via mediating RECK. |
topic |
Ovarian cancer Dihydroartemisinin RECK Apoptosis Invasion |
url |
http://www.sciencedirect.com/science/article/pii/S1347861321000128 |
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