EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE
Objective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome caused by letrozole in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Anatomy department, Army Medical Colle...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Army Press
2021-02-01
|
Series: | Pakistan Armed Forces Medical Journal |
Subjects: | |
Online Access: | https://pafmj.org/index.php/PAFMJ/article/view/3693 |
id |
doaj-41a3a84006c346d7ba6818cda0869025 |
---|---|
record_format |
Article |
spelling |
doaj-41a3a84006c346d7ba6818cda08690252021-09-01T16:38:02ZengThe Army PressPakistan Armed Forces Medical Journal0030-96482411-88422021-02-01711338342https://doi.org/10.51253/pafmj.v71i1.3693EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICENoreen Anwar0Shabnam Hamid1Amer Nadeem2Ayesha Asad3Naureen Waseem4Nomana Mehmood5Quetta Institute of Medical Sciences, Quetta/National University of Medical Sciences (NUMS) PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanCombined Military Hospital, Quetta/ National University of Medical Sciences (NUMS) PakistanQuetta Institute of Medical Sciences, Quetta National University of Medical Sciences (NUMS) PakistanIslamic Medical College, Sialkot PakistanWah Medical College, Wah National University of Medical Sciences (NUMS) PakistanObjective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome caused by letrozole in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Anatomy department, Army Medical College in teamwork with National Institute of Health. Time span is Dec 2014 Dec 2015. Methodology: Total 40 mature female BALB/c mice were chosen and distributed in four groups (ten mice in each one). Group 1 was given normal diet. Rests of three groups were treated with Letrozole at dose of 1 mg/kg body weight once daily for eight weeks. Group 3 was also given Nigella sativa seeds powder at dose of 10 g/kg body weight once daily starting at 22 day and continued up to eight weeks. Group 4 was treated with Nigella sativa oil at a dose of 4 ml/kg body weight once daily starting at 22 day and continued up to eight weeks. At termination of trial, fasting blood glucose level (FBG) was recorded by taking blood sample by glucometer from mouse lateral tail vein after 12 hours fasting. Mice with blood glucose level 250-464 mg/dl were marked hyperglycemic. Final blood sample was taken through cardiac puncture for serum testosterone evaluation. Results: Fasting blood glucose and Serum testosterone levels were raised in group 2 animals in comparison to group 1 while they were reduced remarkably in group 3 and 4 in comparison of group 2. Conclusion: Nigella sativa seeds in both solid and liquid state leave an identical curative impact on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome in mice.https://pafmj.org/index.php/PAFMJ/article/view/3693fasting blood glucoseletrozolenigella sativapolycystic ovarian syndromeserum testosterone level |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Noreen Anwar Shabnam Hamid Amer Nadeem Ayesha Asad Naureen Waseem Nomana Mehmood |
spellingShingle |
Noreen Anwar Shabnam Hamid Amer Nadeem Ayesha Asad Naureen Waseem Nomana Mehmood EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE Pakistan Armed Forces Medical Journal fasting blood glucose letrozole nigella sativa polycystic ovarian syndrome serum testosterone level |
author_facet |
Noreen Anwar Shabnam Hamid Amer Nadeem Ayesha Asad Naureen Waseem Nomana Mehmood |
author_sort |
Noreen Anwar |
title |
EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE |
title_short |
EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE |
title_full |
EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE |
title_fullStr |
EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE |
title_full_unstemmed |
EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE |
title_sort |
effect of nigella sativa on serum testosterone levels in letrozole induced polycystic ovarian syndrome in mice |
publisher |
The Army Press |
series |
Pakistan Armed Forces Medical Journal |
issn |
0030-9648 2411-8842 |
publishDate |
2021-02-01 |
description |
Objective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome caused by letrozole in mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: Anatomy department, Army Medical College in teamwork with National Institute of Health. Time span is Dec 2014 Dec 2015. Methodology: Total 40 mature female BALB/c mice were chosen and distributed in four groups (ten mice in each one). Group 1 was given normal diet. Rests of three groups were treated with Letrozole at dose of 1 mg/kg body weight once daily for eight weeks. Group 3 was also given Nigella sativa seeds powder at dose of 10 g/kg body weight once daily starting at 22 day and continued up to eight weeks. Group 4 was treated with Nigella sativa oil at a dose of 4 ml/kg body weight once daily starting at 22 day and continued up to eight weeks. At termination of trial, fasting blood glucose level (FBG) was recorded by taking blood sample by glucometer from mouse lateral tail vein after 12 hours fasting. Mice with blood glucose level 250-464 mg/dl were marked hyperglycemic. Final blood sample was taken through cardiac puncture for serum testosterone evaluation. Results: Fasting blood glucose and Serum testosterone levels were raised in group 2 animals in comparison to group 1 while they were reduced remarkably in group 3 and 4 in comparison of group 2. Conclusion: Nigella sativa seeds in both solid and liquid state leave an identical curative impact on fasting blood glucose and serum testosterone levels in polycystic ovarian syndrome in mice. |
topic |
fasting blood glucose letrozole nigella sativa polycystic ovarian syndrome serum testosterone level |
url |
https://pafmj.org/index.php/PAFMJ/article/view/3693 |
work_keys_str_mv |
AT noreenanwar effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice AT shabnamhamid effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice AT amernadeem effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice AT ayeshaasad effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice AT naureenwaseem effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice AT nomanamehmood effectofnigellasativaonserumtestosteronelevelsinletrozoleinducedpolycysticovariansyndromeinmice |
_version_ |
1721182325317828608 |