Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the...

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Main Authors: Denis Rwabiita Mugizi, Shaman Muradrasoli, Sofia Boqvist, Joseph Erume, George William Nasinyama, Charles Waiswa, Gerald Mboowa, Markus Klint, Ulf Magnusson
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2015/720413
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spelling doaj-41cf5469e21f46e0aebc88992551dd952020-11-24T22:30:30ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/720413720413Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in UgandaDenis Rwabiita Mugizi0Shaman Muradrasoli1Sofia Boqvist2Joseph Erume3George William Nasinyama4Charles Waiswa5Gerald Mboowa6Markus Klint7Ulf Magnusson8College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, UgandaDivision of Bacteriology and Food Safety, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7028, 75007 Uppsala, SwedenDivision of Bacteriology and Food Safety, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7028, 75007 Uppsala, SwedenCollege of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, UgandaCollege of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, UgandaCollege of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, UgandaCollege of Health Sciences, Makerere University, P.O. Box 7062, Kampala, UgandaSection of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, 75185 Uppsala, SwedenDivision of Reproduction, Department of Clinical Sciences, Faculty of Veterinary, Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7054, 75007 Uppsala, SwedenBrucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.http://dx.doi.org/10.1155/2015/720413
collection DOAJ
language English
format Article
sources DOAJ
author Denis Rwabiita Mugizi
Shaman Muradrasoli
Sofia Boqvist
Joseph Erume
George William Nasinyama
Charles Waiswa
Gerald Mboowa
Markus Klint
Ulf Magnusson
spellingShingle Denis Rwabiita Mugizi
Shaman Muradrasoli
Sofia Boqvist
Joseph Erume
George William Nasinyama
Charles Waiswa
Gerald Mboowa
Markus Klint
Ulf Magnusson
Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
BioMed Research International
author_facet Denis Rwabiita Mugizi
Shaman Muradrasoli
Sofia Boqvist
Joseph Erume
George William Nasinyama
Charles Waiswa
Gerald Mboowa
Markus Klint
Ulf Magnusson
author_sort Denis Rwabiita Mugizi
title Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
title_short Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
title_full Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
title_fullStr Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
title_full_unstemmed Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
title_sort isolation and molecular characterization of brucella isolates in cattle milk in uganda
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2015-01-01
description Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.
url http://dx.doi.org/10.1155/2015/720413
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