Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats
In the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg...
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2020-03-01
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doaj-41f195794b1045e5908283bcd9c11c212021-05-05T07:28:15ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142020-03-01711e345e34510.3989/gya.11751821781Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in ratsS. M. Galal0https://orcid.org/0000-0002-1859-1641M. K.S. Morsi1https://orcid.org/0000-0003-2061-6075M. K. Abd El-Rahman2https://orcid.org/0000-0003-0474-3023S. K. Darwish3https://orcid.org/0000-0001-7480-160XM. A. Katry4https://orcid.org/0000-0002-2857-671XDepartment of Food Science, Faculty of Agriculture, Cairo UniversityDepartment of Food Science, Faculty of Agriculture, Cairo UniversityNational Nutrition Institute, Ministry of Health and PopulationNational Organization for Drug Control and Research, Ministry of Health and PopulationNational Nutrition Institute, Ministry of Health and PopulationIn the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg/mL). The constituents of UNSAP were determined by GC-MS. The subcutaneous administration of CCl4, caused liver injury. The hepatoprotective effect of UNSAP was comparable to that of α-tocopherol, a standard antioxidant agent. The co-administration of the investigated UNSAP normalized the activities of serum marker enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Furthermore, the serum alkaline phosphatase (ALP) activity and hepatic malondialdehyde (MDA) level were found to be alleviated by pre-treatment with the UNSAP. A histopathological evaluation showed marked improvement in the liver of UNSAP- and α-Tocopherol-treated animals. The hepatoprotective effect could be attributed to the antioxidant characteristics of UNSAP.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1815ccl4hepatotoxicityhistopathologyratunsaponifiable matter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. M. Galal M. K.S. Morsi M. K. Abd El-Rahman S. K. Darwish M. A. Katry |
spellingShingle |
S. M. Galal M. K.S. Morsi M. K. Abd El-Rahman S. K. Darwish M. A. Katry Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats Grasas y Aceites ccl4 hepatotoxicity histopathology rat unsaponifiable matter |
author_facet |
S. M. Galal M. K.S. Morsi M. K. Abd El-Rahman S. K. Darwish M. A. Katry |
author_sort |
S. M. Galal |
title |
Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
title_short |
Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
title_full |
Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
title_fullStr |
Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
title_full_unstemmed |
Hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
title_sort |
hepatoprotective effect of the unsaponifiable matter from olive, linseed and sesame oils against carbon tetrachloride-induced liver injury in rats |
publisher |
Consejo Superior de Investigaciones Científicas |
series |
Grasas y Aceites |
issn |
0017-3495 1988-4214 |
publishDate |
2020-03-01 |
description |
In the present study, the hepatoprotective activity of the unsaponifiable matter (UNSAP) of olive oil, linseed, and sesame oils against CCl4-induced liver toxicity in rats was investigated. In a preliminary antioxidant study, UNSAP showed pronounced DPPH radical scavenging activity (IC50 6.2-10.8 mg/mL). The constituents of UNSAP were determined by GC-MS. The subcutaneous administration of CCl4, caused liver injury. The hepatoprotective effect of UNSAP was comparable to that of α-tocopherol, a standard antioxidant agent. The co-administration of the investigated UNSAP normalized the activities of serum marker enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Furthermore, the serum alkaline phosphatase (ALP) activity and hepatic malondialdehyde (MDA) level were found to be alleviated by pre-treatment with the UNSAP. A histopathological evaluation showed marked improvement in the liver of UNSAP- and α-Tocopherol-treated animals. The hepatoprotective effect could be attributed to the antioxidant characteristics of UNSAP. |
topic |
ccl4 hepatotoxicity histopathology rat unsaponifiable matter |
url |
http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1815 |
work_keys_str_mv |
AT smgalal hepatoprotectiveeffectoftheunsaponifiablematterfromolivelinseedandsesameoilsagainstcarbontetrachlorideinducedliverinjuryinrats AT mksmorsi hepatoprotectiveeffectoftheunsaponifiablematterfromolivelinseedandsesameoilsagainstcarbontetrachlorideinducedliverinjuryinrats AT mkabdelrahman hepatoprotectiveeffectoftheunsaponifiablematterfromolivelinseedandsesameoilsagainstcarbontetrachlorideinducedliverinjuryinrats AT skdarwish hepatoprotectiveeffectoftheunsaponifiablematterfromolivelinseedandsesameoilsagainstcarbontetrachlorideinducedliverinjuryinrats AT makatry hepatoprotectiveeffectoftheunsaponifiablematterfromolivelinseedandsesameoilsagainstcarbontetrachlorideinducedliverinjuryinrats |
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1721470314450255872 |