Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death
Triple negative breast cancer (TNBC) represents an aggressive phenotype with poor prognosis compared with ER, PR, and HER2‐positive tumors. TNBC is a heterogeneous disease, and gene expression analysis has identified seven molecular subtypes. Accumulating evidence demonstrates that long non‐coding R...
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doaj-420afe31547c4bf396ed619abbe1e1c72020-11-25T03:18:17ZengWileyMolecular Oncology1574-78911878-02612019-04-0113490992710.1002/1878-0261.12446Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell deathFredy Omar Beltrán‐Anaya0Sandra Romero‐Córdoba1Rosa Rebollar‐Vega2Oscar Arrieta3Verónica Bautista‐Piña4Carlos Dominguez‐Reyes5Felipe Villegas‐Carlos6Alberto Tenorio‐Torres7Luis Alfaro‐Riuz8Silvia Jiménez‐Morales9Alberto Cedro‐Tanda10Magdalena Ríos‐Romero11Juan Pablo Reyes‐Grajeda12Elda Tagliabue13Marilena V. Iorio14Alfredo Hidalgo‐Miranda15Laboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoThoracic Oncology Unit Instituto Nacional de Cancerología (INCan) Mexico City MexicoInstituto de Enfermedades de la Mama FUCAM Mexico City MexicoInstituto de Enfermedades de la Mama FUCAM Mexico City MexicoInstituto de Enfermedades de la Mama FUCAM Mexico City MexicoInstituto de Enfermedades de la Mama FUCAM Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoUnidad de Proteómica Médica Instituto Nacional de Medicina Genómica Mexico City MexicoDepartment of Experimental Oncology and Molecular Medicine Istituto Nazionale dei Tumori Milan ItalyDepartment of Experimental Oncology and Molecular Medicine Istituto Nazionale dei Tumori Milan ItalyLaboratorio de Genómica del Cáncer Instituto Nacional de Medicina Genómica Mexico City MexicoTriple negative breast cancer (TNBC) represents an aggressive phenotype with poor prognosis compared with ER, PR, and HER2‐positive tumors. TNBC is a heterogeneous disease, and gene expression analysis has identified seven molecular subtypes. Accumulating evidence demonstrates that long non‐coding RNA (lncRNA) are involved in regulation of gene expression and cancer biology, contributing to essential cancer cell functions. In this study, we analyzed the expression profile of lncRNA in TNBC subtypes from 156 TNBC samples, and then characterized the functional role of LncKLHDC7B (ENSG00000226738). A total of 710 lncRNA were found to be differentially expressed between TNBC subtypes, and a subset of these altered lncRNA were independently validated. We discovered that LncKLHDC7B (ENSG00000226738) acts as a transcriptional modulator of its neighboring coding gene KLHDC7B in the immunomodulatory subtype. Furthermore, LncKLHDC7B knockdown enhanced migration and invasion, and promoted resistance to cellular death. Our findings confirmed the contribution of LncKLHDC7B to induction of apoptosis and inhibition of cell migration and invasion, suggesting that TNBC tumors with enrichment of LncKLHDC7B may exhibit distinct regulatory activity, or that this may be a generalized process in breast cancer. Additionally, in silico analysis confirmed for the first time that the low expression of KLHDC7B and LncKLHDC7B is associated with poor prognosis in patients with breast cancer.https://doi.org/10.1002/1878-0261.12446ENSG00000226738invasionLncKLHDC7Blong non‐coding RNAmigrationtriple‐negative breast cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fredy Omar Beltrán‐Anaya Sandra Romero‐Córdoba Rosa Rebollar‐Vega Oscar Arrieta Verónica Bautista‐Piña Carlos Dominguez‐Reyes Felipe Villegas‐Carlos Alberto Tenorio‐Torres Luis Alfaro‐Riuz Silvia Jiménez‐Morales Alberto Cedro‐Tanda Magdalena Ríos‐Romero Juan Pablo Reyes‐Grajeda Elda Tagliabue Marilena V. Iorio Alfredo Hidalgo‐Miranda |
spellingShingle |
Fredy Omar Beltrán‐Anaya Sandra Romero‐Córdoba Rosa Rebollar‐Vega Oscar Arrieta Verónica Bautista‐Piña Carlos Dominguez‐Reyes Felipe Villegas‐Carlos Alberto Tenorio‐Torres Luis Alfaro‐Riuz Silvia Jiménez‐Morales Alberto Cedro‐Tanda Magdalena Ríos‐Romero Juan Pablo Reyes‐Grajeda Elda Tagliabue Marilena V. Iorio Alfredo Hidalgo‐Miranda Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death Molecular Oncology ENSG00000226738 invasion LncKLHDC7B long non‐coding RNA migration triple‐negative breast cancer |
author_facet |
Fredy Omar Beltrán‐Anaya Sandra Romero‐Córdoba Rosa Rebollar‐Vega Oscar Arrieta Verónica Bautista‐Piña Carlos Dominguez‐Reyes Felipe Villegas‐Carlos Alberto Tenorio‐Torres Luis Alfaro‐Riuz Silvia Jiménez‐Morales Alberto Cedro‐Tanda Magdalena Ríos‐Romero Juan Pablo Reyes‐Grajeda Elda Tagliabue Marilena V. Iorio Alfredo Hidalgo‐Miranda |
author_sort |
Fredy Omar Beltrán‐Anaya |
title |
Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
title_short |
Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
title_full |
Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
title_fullStr |
Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
title_full_unstemmed |
Expression of long non‐coding RNA ENSG00000226738 (LncKLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
title_sort |
expression of long non‐coding rna ensg00000226738 (lncklhdc7b) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death |
publisher |
Wiley |
series |
Molecular Oncology |
issn |
1574-7891 1878-0261 |
publishDate |
2019-04-01 |
description |
Triple negative breast cancer (TNBC) represents an aggressive phenotype with poor prognosis compared with ER, PR, and HER2‐positive tumors. TNBC is a heterogeneous disease, and gene expression analysis has identified seven molecular subtypes. Accumulating evidence demonstrates that long non‐coding RNA (lncRNA) are involved in regulation of gene expression and cancer biology, contributing to essential cancer cell functions. In this study, we analyzed the expression profile of lncRNA in TNBC subtypes from 156 TNBC samples, and then characterized the functional role of LncKLHDC7B (ENSG00000226738). A total of 710 lncRNA were found to be differentially expressed between TNBC subtypes, and a subset of these altered lncRNA were independently validated. We discovered that LncKLHDC7B (ENSG00000226738) acts as a transcriptional modulator of its neighboring coding gene KLHDC7B in the immunomodulatory subtype. Furthermore, LncKLHDC7B knockdown enhanced migration and invasion, and promoted resistance to cellular death. Our findings confirmed the contribution of LncKLHDC7B to induction of apoptosis and inhibition of cell migration and invasion, suggesting that TNBC tumors with enrichment of LncKLHDC7B may exhibit distinct regulatory activity, or that this may be a generalized process in breast cancer. Additionally, in silico analysis confirmed for the first time that the low expression of KLHDC7B and LncKLHDC7B is associated with poor prognosis in patients with breast cancer. |
topic |
ENSG00000226738 invasion LncKLHDC7B long non‐coding RNA migration triple‐negative breast cancer |
url |
https://doi.org/10.1002/1878-0261.12446 |
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