Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip
Abstract Background A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be...
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Format: | Article |
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BMC
2019-02-01
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Series: | Journal of Nanobiotechnology |
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Online Access: | http://link.springer.com/article/10.1186/s12951-019-0458-2 |
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doaj-420c4df5d1e849a990f164b73d952b28 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Li Chuanlin Hu Pengcheng Wang Yan Liu Luyang Wang Qingmeng Pi Zhiyong Gong Xu Yang Michael Mak Yang Wu |
spellingShingle |
Yan Li Chuanlin Hu Pengcheng Wang Yan Liu Luyang Wang Qingmeng Pi Zhiyong Gong Xu Yang Michael Mak Yang Wu Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip Journal of Nanobiotechnology Indoor particulate matter Oxidative stress Inflammation Promote coagulation 3D human microvessel |
author_facet |
Yan Li Chuanlin Hu Pengcheng Wang Yan Liu Luyang Wang Qingmeng Pi Zhiyong Gong Xu Yang Michael Mak Yang Wu |
author_sort |
Yan Li |
title |
Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip |
title_short |
Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip |
title_full |
Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip |
title_fullStr |
Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip |
title_full_unstemmed |
Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip |
title_sort |
indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3d microvascular model on a microfluidic chip |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2019-02-01 |
description |
Abstract Background A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be explored. Based on animal models, ambient particulate matter has been proved to promote coagulation which is very likely involved in the pathogenic development of cardiovascular disease. However, animal models are insufficient to predict what will happen with any certainty in humans. For this reason, the precise pathogenic mechanisms behind the development of cardiovascular disease in humans have not yet been determined. Results We generated a 3D functional human microvascular network in a microfluidic device. This model enables human vascular endothelial cells to form tissue-like microvessels that behave very similarly to human blood vessels. The perfusable microvasculature allows the delivery of particles introduced into these generated human-like microvessels to follow the fluid flow. This exposure path effectively simulates the dynamic movement of airborne nanoscale particles (ANPs) within human vessels. In this study, we first identified the existence of ANPs in indoor air pollution. We then showed that ANPs could activate endothelial cells via ROS induced inflammation, and further resulted in abnormal expression of the coagulation factors (TF, TM and t-PA) involved in coagulation cascades. In addition, we found that a protein could cover ANPs, and this biointeraction could interfere with heparan sulfate (HS). Human organotypic 3D microvessel models provide a bridge for how research outcomes can translate to humans. Conclusions The 3D human microvessel model was used to determine the physiological responses of human vessels to ANP stimulation. Based on the obtained data, we concluded that ANPs not only disrupts normal coagulation functions, but also act directly on anticoagulant factors in human vessels. These experimental observations provide a potential biological explanation for the epidemiologically established link between ANPs and coagulation abnormality. This organ-on-chip model may provide a bridge from in vitro results to human responses. |
topic |
Indoor particulate matter Oxidative stress Inflammation Promote coagulation 3D human microvessel |
url |
http://link.springer.com/article/10.1186/s12951-019-0458-2 |
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doaj-420c4df5d1e849a990f164b73d952b282020-11-25T02:17:06ZengBMCJournal of Nanobiotechnology1477-31552019-02-0117111710.1186/s12951-019-0458-2Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chipYan Li0Chuanlin Hu1Pengcheng Wang2Yan Liu3Luyang Wang4Qingmeng Pi5Zhiyong Gong6Xu Yang7Michael Mak8Yang Wu9Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic UniversityState Key Laboratory of Silicate Materials for Architectures, Wuhan University of TechnologyKey Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic UniversityKey Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic UniversityDepartment of Building Science, Tsinghua UniversityHarvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of TechnologyKey Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal UniversityDepartment of Biomedical Engineering, School of Engineering & Applied Science, Yale UniversityHubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), College of Food Science and Engineering, Wuhan Polytechnic UniversityAbstract Background A growing body of evidence shows that indoor concentrations of airborne particles are often higher than is typically encountered outdoors. Since exposure to indoor PM2.5 is thought to be associated with cardiovascular disease, the health impacts of indoor air pollution need to be explored. Based on animal models, ambient particulate matter has been proved to promote coagulation which is very likely involved in the pathogenic development of cardiovascular disease. However, animal models are insufficient to predict what will happen with any certainty in humans. For this reason, the precise pathogenic mechanisms behind the development of cardiovascular disease in humans have not yet been determined. Results We generated a 3D functional human microvascular network in a microfluidic device. This model enables human vascular endothelial cells to form tissue-like microvessels that behave very similarly to human blood vessels. The perfusable microvasculature allows the delivery of particles introduced into these generated human-like microvessels to follow the fluid flow. This exposure path effectively simulates the dynamic movement of airborne nanoscale particles (ANPs) within human vessels. In this study, we first identified the existence of ANPs in indoor air pollution. We then showed that ANPs could activate endothelial cells via ROS induced inflammation, and further resulted in abnormal expression of the coagulation factors (TF, TM and t-PA) involved in coagulation cascades. In addition, we found that a protein could cover ANPs, and this biointeraction could interfere with heparan sulfate (HS). Human organotypic 3D microvessel models provide a bridge for how research outcomes can translate to humans. Conclusions The 3D human microvessel model was used to determine the physiological responses of human vessels to ANP stimulation. Based on the obtained data, we concluded that ANPs not only disrupts normal coagulation functions, but also act directly on anticoagulant factors in human vessels. These experimental observations provide a potential biological explanation for the epidemiologically established link between ANPs and coagulation abnormality. This organ-on-chip model may provide a bridge from in vitro results to human responses.http://link.springer.com/article/10.1186/s12951-019-0458-2Indoor particulate matterOxidative stressInflammationPromote coagulation3D human microvessel |